[化学诱导激活标记拟南芥T-DNA插入系的产生]。

Jian Zhang, Jin-Xiang Xu, Ying-Zhen Kong, Zhen-Dong Ji, Xing-Chun Wang, Feng-Ying An, Chao Li, Jia-Qiang Sun, Su-Zhi Zhang, Xiao-Hui Yang, Jin-Ye Mu, Xin-Fang Liu, Jia-Yang Li, Yong-Biao Xue, Jian-Ru Zuo
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引用次数: 0

摘要

利用雌激素诱导表达的XVE (LexA-VP16-Estragon Receptor)系统,我们已经生成了大约40000个独立的拟南芥T-DNA插入系。对18000份样品的分离分析表明,51.6%的样品含有单个T-DNA插入,平均插入数为1.38个拷贝/条。发现了多种形态改变的突变体,包括影响根、下胚轴、叶片、花器官和种子的发育以及开花时间的突变体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana].

Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18000 lines indicated that 51.6% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots,hypocotyls, leaves, floral organs and seeds as well as the flowering time.

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