意大利蜜蜂蜂王浆生产性状的微卫星分析。

Sheng-Lu Chen, Jian-Ke Li, Bo-Xiong Zhong, Song-Kun Su
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引用次数: 0

摘要

通过10个微卫星位点的遗传变异分析,确定了意大利本土蜜蜂(Ee)、中国-意大利蜜蜂(Eb)和精选高产蜂王浆蜜蜂(Ea) 3个意大利和中国蜜蜂(Apis mellifera L.)种群的进化关系和分子特征。在Ee、Eb和Ea蜜蜂的10个位点共检测到96个等位基因,平均每个位点9.6个等位基因。其中48例(5%)存在差异。这表明三个居群之间存在高度的多态性,但由于人为选择和地理隔离的原因,存在一定的遗传分化。3个群体在10个位点上的多态性信息含量(PIC)和杂合度分别为0.57、0.50、0.57和0.60、0.57、0.61,差异均无统计学意义。这表明三个群体的基因多样性相同。Ee蜂与Eb蜂、Eb蜂与Ea蜂的遗传距离较短。而Ee和Eb蜜蜂之间的时间更长。进一步分析表明,A29,100 bp和A24位点的等位基因频率分别为159 bp和104 bp;A7处110 bp;A43、A14和A113位点分别增加了126 bp、221 bp和221 bp。配对试验表明,Ea蜜蜂和Eb蜜蜂之间以及Ea蜜蜂和Eb蜜蜂之间的等位基因频率显著高于Eb蜜蜂。这表明这7个等位基因可能是高产蜂王浆蜜蜂的分子标记。此外,4个位点上的4个等位基因频率(a24106 bp, A43 140 bp, A113 215 bp, A14 219 bp)从Ea蜜蜂到Eb蜜蜂再到Ee蜜蜂均呈下降趋势。配对试验表明,Ea蜜蜂和Ee蜜蜂以及Ea蜜蜂和Eb蜜蜂之间的等位基因频率显著降低。这4个等位基因可能是蜂王浆产量低的遗传标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microsatellite analysis of royal jelly producing traits of Italian honeybee (Apis mellifera Liguatica).

Genetic variations at 10 microsatellite loci were surveyed to determine the evolutionary relationships and molecular characteristics of three different honeybee (Apis mellifera L.) populations from Italy and China, i. e., native Italian (Ee), Chinese-Italian (Eb) and selected high royal jelly producing bees (Ea). A total of 96 alleles,an average of 9.6 alleles per locus,were scored in Ee,Eb and Ea bees at 10 loci. Out of which 48 (5%) were different. This indicated a high degree of polymorphism and ever, some genetic differentiation among the three populations due to artificial selection and geographical isolation. The polymorphic information contents (PIC) and heterozyosity of the three populations at 10 loci were 0.57, 0.50, 0.57, and 0.60, 0.57, 0.61, for Ee, Eb, Ea populations respectively, neither of which were different. This indicated same gene diversity within the three populations. The genetic distance was shorter between Ee and Eb bees as well as between Eb and Ea bees. Whereas that between Ee and Eb bees was longer. Further analysis indicated that the allele frequency of seven alleles at six loci (159 bp at A29,100 bp and 104 bp at A24; 110 bp at A7; 126 bp at A43, 221 bp at A14 and 221 bp at A113) increased going from Ee to Eb to Ea bees. Paired tests showed significant higher allele frequency between Ea and Eb bees,as well as between Ea and Eb bees. This indicates that these seven alleles are likely molecular markers of the high royal jelly producing bees. In addition,the allele frequency of four alleles at four loci (106 bp at A24,140 bp at A43;215 bp at A113 and 219 bp at A14) decreased going from Ea bees to Eb to Ee. Paired tests indicated significant lower allele frequency between Ea and Ee bees,as well as between Ea and Eb bees. Those four alleles may be the genetic markers for low royal jelly production.

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