转录组阵列筛选和验证与口腔白斑病癌变相关的缺氧反应基因和微RNA

Q3 Medicine

华西口腔医学杂志 Pub Date : 2021-02-01 DOI:10.7518/hxkq.2021.01.003

Lin-Jun Shi, Xi Yang, Su-Ning Wu, Wei Liu

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引用次数: 0

摘要

目的研究缺氧反应基因和microRNA（miRNA）在口腔白斑病（OLK）发病和进展过程中的表达谱：采用Affymetrix GeneChip人类转录组阵列2.0检测正常黏膜、低危OLK、高危OLK和早期鳞状细胞癌（SCC）的转录组。利用基因本体功能分析筛选出在缺氧反应中起生物学作用的基因和关键 miRNA。采用定量反转录聚合酶链式反应（qRT-PCR）验证缺氧反应基因和miRNAs的表达：结果：在正常黏膜和低危 OLK 之间共鉴定出 7 个不同的缺氧反应基因，在低危和高危 OLK 之间共鉴定出 10 个不同的缺氧反应基因，在高危 OLK 和 SCC 之间共鉴定出 21 个不同的缺氧反应基因。qRT-PCR结果显示，缺氧诱导因子1α、趋化因子cc-motif配体2、基质金属蛋白酶3 mRNA和miR-21在正常黏膜、OLK和SCC中的表达呈阶梯状增加。OLK和SCC的表达差异有统计学意义，与转录组阵列的结果一致：结论：缺氧反应基因和相关 miRNA 在 OLK 的发生和发展过程中起着重要作用。

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Transcriptome array screening and verification of oral leukoplakia carcinogenesis-related hypoxia-responsive gene and microRNA.

Objectives: To study the hypoxia response gene and microRNA (miRNA) expression profiles in the pathogenesis and progression of oral leukoplakia (OLK).

Methods: Affymetrix GeneChip human transcriptome array 2.0 was used to detect the transcriptome of normal mucosa, low-risk OLK, high-risk OLK, and early squamous cell carcinoma (SCC). Gene ontology function analysis was used to screen genes and key miRNAs whose biological role is hypoxia response. Quantitative reverse transcription polymerase ch-ain reaction (qRT-PCR) was used to verify the expression of hypoxia response genes and miRNAs.

Results: A total of 7 different genes of hypoxia response between normal mucosa and low-risk OLK, 10 genes between low-risk and high-risk OLK, and 21 genes between high-risk OLK and SCC were identified. The results of qRT-PCR showed that the expression of hypoxia-inducible factor 1α, chemokine cc-motif ligand 2, and matrix metalloproteinase 3 mRNA and miR-21 in normal mucosa, OLK, and SCC increased in a stepwise manner. The expression difference between OLK and SCC was statistically significant and consistent with the results of transcriptome array.

Conclusions: The hypoxia response gene and related miRNA play roles in the development and progression of OLK.

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来源期刊

华西口腔医学杂志 Medicine-Medicine (all)

CiteScore

0.80

自引率

0.00%

发文量

6397

期刊介绍： West China Journal of Stomatology (WCJS, pISSN 1000-1182, eISSN 2618-0456, CN 51-1169/R), published bimonthly, is a peer-reviewed Open Access journal, hosted by Sichuan university and Ministry of Education of the People's Republic of China. WCJS was established in 1983 and indexed in Medline/Pubmed, SCOPUS, EBSCO, Chemical Abstract(CA), CNKI, WANFANG Data, etc.