{"title":"2-[双(羧甲基)氨基]丙酸螯合铜螯合物增强过碳酸钠对细菌的清除作用。","authors":"Urara Ishizaki, Iwahito Takahashi, Katsumi Sato, Kazuaki Yoshimune","doi":"10.4265/bio.26.9","DOIUrl":null,"url":null,"abstract":"<p><p>Sodium percarbonate (SP) is a relatively low-cost and stable solid oxidizer with a small environmental burden. It is often included in cleansers for sanitizing circulating water pipes and as bleaching reagents in laundry, although the bactericidal effect of SP is lower than that of chlorine-based agents. 2-[Bis(carboxymethyl)amino] propanoic acid-chelated copper (MGDA-Cu) was added to increase the effect of SP. The addition of 12 µM MGDA-Cu increased the bactericidal effect of 0.5 wt% SP against Staphylococcus aureus even in the presence of 0.3 wt% BSA, which is an experimental model of organic stain to protect bacteria from SP. MGDA-Cu was effective against Escherichia coli only in the absence of BSA and showed little effect against Bacillus subtilis. It enhanced the effect of SP to decrease the viscosity of sodium alginate, which is one of the major components of biofilms. The effect of MGDA-Cu on sanitization was also evaluated by 16S rRNA amplicon sequencing of the bacterial flora of the biofilm on an experimental model of a circulating water pipe. The structure of the bacterial flora was more influenced by a cleanser containing both MGDA-Cu and SP than a cleanser with only SP, suggesting that MGDA-Cu increases the sanitization effect.</p>","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"26 1","pages":"9-15"},"PeriodicalIF":0.9000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"2-[Bis(carboxymethyl)amino] propanoic acid-Chelated Copper Chelate Enhances Bacterial Elimination by Sodium Percarbonate.\",\"authors\":\"Urara Ishizaki, Iwahito Takahashi, Katsumi Sato, Kazuaki Yoshimune\",\"doi\":\"10.4265/bio.26.9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Sodium percarbonate (SP) is a relatively low-cost and stable solid oxidizer with a small environmental burden. It is often included in cleansers for sanitizing circulating water pipes and as bleaching reagents in laundry, although the bactericidal effect of SP is lower than that of chlorine-based agents. 2-[Bis(carboxymethyl)amino] propanoic acid-chelated copper (MGDA-Cu) was added to increase the effect of SP. The addition of 12 µM MGDA-Cu increased the bactericidal effect of 0.5 wt% SP against Staphylococcus aureus even in the presence of 0.3 wt% BSA, which is an experimental model of organic stain to protect bacteria from SP. MGDA-Cu was effective against Escherichia coli only in the absence of BSA and showed little effect against Bacillus subtilis. It enhanced the effect of SP to decrease the viscosity of sodium alginate, which is one of the major components of biofilms. The effect of MGDA-Cu on sanitization was also evaluated by 16S rRNA amplicon sequencing of the bacterial flora of the biofilm on an experimental model of a circulating water pipe. The structure of the bacterial flora was more influenced by a cleanser containing both MGDA-Cu and SP than a cleanser with only SP, suggesting that MGDA-Cu increases the sanitization effect.</p>\",\"PeriodicalId\":8777,\"journal\":{\"name\":\"Biocontrol science\",\"volume\":\"26 1\",\"pages\":\"9-15\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biocontrol science\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.4265/bio.26.9\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocontrol science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.4265/bio.26.9","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 1
摘要
过碳酸钠(SP)是一种低成本、稳定、环境负担小的固体氧化剂。虽然SP的杀菌效果低于氯基药剂,但它通常被加入循环水管消毒的清洁剂中,并在洗衣房中用作漂白剂。添加2-[双(羧甲基)氨基]丙酸螯合铜(MGDA-Cu)以提高SP的效果。添加12 μ M MGDA-Cu后,即使在0.3 wt% BSA存在的情况下,也能提高0.5 wt% SP对金黄色葡萄球菌的杀菌效果,这是一种有机染色保护细菌免受SP感染的实验模型。MGDA-Cu仅在不存在BSA的情况下对大肠杆菌有效,对枯草芽孢杆菌的作用很小。海藻酸钠是生物膜的主要组成成分之一,它可以增强SP降低海藻酸钠粘度的作用。通过对循环水管实验模型生物膜菌群的16S rRNA扩增子测序,评价了MGDA-Cu的消毒效果。同时含有MGDA-Cu和SP的清洁剂比只含有SP的清洁剂对细菌菌群结构的影响更大,表明MGDA-Cu增加了消毒效果。
Sodium percarbonate (SP) is a relatively low-cost and stable solid oxidizer with a small environmental burden. It is often included in cleansers for sanitizing circulating water pipes and as bleaching reagents in laundry, although the bactericidal effect of SP is lower than that of chlorine-based agents. 2-[Bis(carboxymethyl)amino] propanoic acid-chelated copper (MGDA-Cu) was added to increase the effect of SP. The addition of 12 µM MGDA-Cu increased the bactericidal effect of 0.5 wt% SP against Staphylococcus aureus even in the presence of 0.3 wt% BSA, which is an experimental model of organic stain to protect bacteria from SP. MGDA-Cu was effective against Escherichia coli only in the absence of BSA and showed little effect against Bacillus subtilis. It enhanced the effect of SP to decrease the viscosity of sodium alginate, which is one of the major components of biofilms. The effect of MGDA-Cu on sanitization was also evaluated by 16S rRNA amplicon sequencing of the bacterial flora of the biofilm on an experimental model of a circulating water pipe. The structure of the bacterial flora was more influenced by a cleanser containing both MGDA-Cu and SP than a cleanser with only SP, suggesting that MGDA-Cu increases the sanitization effect.
期刊介绍:
The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.