丹参化学纯化合物的最佳组合抑制细胞增殖。

Q2 Medicine
Tiemei Shao, Jing Zhang, Shengjun An, Hongjun Xu, Jiangli Wu, Lei Wang, Wei Zhao, Yongjie Zhou, Luyang Zhou, Qingzhuo Cui
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引用次数: 1

摘要

目的:丹参是临床上常用的治疗心血管疾病的中药,包括动脉粥样硬化和心肌梗死。其治疗效果已被许多临床和药理学研究证实。然而,SM治疗心血管疾病的有效成分的最佳配方尚不清楚。本研究采用均匀正交优化实验设计,确定了SM成分DSS、Sal-A、Sal-B、PAL (SABP)的最佳配伍比例。此外,我们利用外基质成纤维细胞(AFs)测定了SABP的抗氧化作用。方法:采用均匀设计和正交设计相结合的方法,确定中药水提液的最佳配方。采用MTT法测定SM 4种成分对主动脉分离培养的AFs的抑制作用。比较了SABP处理前后AFs的活性氧(ROS)生成情况。结果:四种水提物的最佳处方为150:7:300:500,其浓度分别为S(1.5×10-4 mol/L)、A(7×10-6 mol/L)、B(3×10-4 mol/L)、P(5×10-4 mol/L)。这4种成分之间存在一定的协同效应。此外,SABP降低了AFs中ROS的产生。结论:SABP具有抑制AFs增殖和氧化应激的作用。本研究提供了新的证据,证明SM中活性成分的最佳配方所产生的功效和功能优于SM冻干粉。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An Optimal Combination of Chemically Pure Compounds from Salvia miltiorrhiza for Inhibiting Cell Proliferation.

Objective: Salvia miltiorrhiza (SM) is a traditional Chinese medicine used clinically to treat cardiovascular diseases, including atherosclerosis and myocardial infarction. Its therapeutic effect has been confirmed by many clinical and pharmacological studies. However, the optimal formulation of active ingredients in SM for treating cardiovascular diseases remains unclear. In this study, we determined the ratio of the optimal compatibility of SM ingredients DSS, Sal-A, Sal-B, and PAL (SABP)with a uniform and orthogonal optimized experimental design. In addition, we determined the anti-oxidation effect of SABP using Adventitial Fibroblasts (AFs).

Methods: By using a combination of uniform and orthogonal designs, we determined the optimal formulation of aqueous extract from SM. MTT assay was used to determine the inhibitory effects of these 4 components of SM on the AFs, which were isolated and cultured from the aorta. The reactive oxygen species (ROS) production in AFs was compared before and after SABP treatment.

Results: The optimal formulation of these 4 aqueous extracts from SM were 150 : 7 : 300 : 500, and their concentrations were S(1.5×10-4 mol/L), A(7×10-6 mol/L), B(3×10-4 mol/L), and P(5×10-4 mol/L). There were some synergies between these 4 components. Moreover, SABP decreased ROS production in AFs.

Conclusion: These findings suggest that SABP inhibits the proliferation and oxidation stress in AFs. The present study provides new evidence that the efficacy and function generated from the optimal formulation of active ingredients in SM are better than lyophilized powder of SM.

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来源期刊
Cardiovascular and Hematological Agents in Medicinal Chemistry
Cardiovascular and Hematological Agents in Medicinal Chemistry Medicine-Cardiology and Cardiovascular Medicine
CiteScore
2.70
自引率
0.00%
发文量
34
期刊介绍: Cardiovascular & Hematological Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new Cardiovascular & Hematological Agents. Each issue contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in Cardiovascular & Hematological medicinal chemistry. Cardiovascular & Hematological Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cardiovascular & hematological drug discovery.
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