从酿酒酵母核糖体谱推断翻译异质性。

IF 5.5 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
FEBS Journal Pub Date : 2021-08-01 Epub Date: 2021-02-22 DOI:10.1111/febs.15748
Pedro do Couto Bordignon, Sebastian Pechmann
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引用次数: 1

摘要

核糖体将mrna翻译成蛋白质是蛋白质生物合成过程中最重要的一步。因此,翻译受到严格控制和严格调节,以维持细胞内稳态。核糖体分析(Ribo-seq)通过揭示翻译的许多潜在机制,彻底改变了翻译的研究。然而,翻译的许多方面仍然是神秘的,部分原因也在于对从核糖核酸测序实验中获得的数据的解释一直存在挑战。在这里,我们表明在核糖核酸序列数据中观察到的一些变异具有生物学起源,反映了翻译的程序化异质性。通过对酿酒酵母(Saccharomyces cerevisiae)的核糖核酸序列(Ribo-seq)数据的比较分析,我们系统地鉴定了跨mrna差异翻译(DT)的短3密码子序列,即相同的序列,有时快,有时慢,超出了实验之间的可变性。值得注意的是,由此确定的DT序列与已知的调节翻译伸长的机制有关,并且富含对蛋白质和细胞器生物合成重要的基因。因此,我们的研究结果突出了翻译异质性的例子,这些异质性被编码在基因组序列中,并调整为优化细胞稳态。更一般地说,我们的工作强调了Ribo-seq在理解翻译调控的复杂性方面的力量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inferring translational heterogeneity from Saccharomyces cerevisiae ribosome profiling.

Translation of mRNAs into proteins by the ribosome is the most important step of protein biosynthesis. Accordingly, translation is tightly controlled and heavily regulated to maintain cellular homeostasis. Ribosome profiling (Ribo-seq) has revolutionized the study of translation by revealing many of its underlying mechanisms. However, equally many aspects of translation remain mysterious, in part also due to persisting challenges in the interpretation of data obtained from Ribo-seq experiments. Here, we show that some of the variability observed in Ribo-seq data has biological origins and reflects programmed heterogeneity of translation. Through a comparative analysis of Ribo-seq data from Saccharomyces cerevisiae, we systematically identify short 3-codon sequences that are differentially translated (DT) across mRNAs, that is, identical sequences that are translated sometimes fast and sometimes slowly beyond what can be attributed to variability between experiments. Remarkably, the thus identified DT sequences link to mechanisms known to regulate translation elongation and are enriched in genes important for protein and organelle biosynthesis. Our results thus highlight examples of translational heterogeneity that are encoded in the genomic sequences and tuned to optimizing cellular homeostasis. More generally, our work highlights the power of Ribo-seq to understand the complexities of translation regulation.

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来源期刊
FEBS Journal
FEBS Journal 生物-生化与分子生物学
CiteScore
11.70
自引率
1.90%
发文量
375
审稿时长
1 months
期刊介绍: The FEBS Journal is an international journal devoted to the rapid publication of full-length papers covering a wide range of topics in any area of the molecular life sciences. The criteria for acceptance are originality and high quality research, which will provide novel perspectives in a specific area of research, and will be of interest to our broad readership. The journal does not accept papers that describe the expression of specific genes and proteins or test the effect of a drug or reagent, without presenting any biological significance. Papers describing bioinformatics, modelling or structural studies of specific systems or molecules should include experimental data.
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