水通道蛋白-9在大鼠体内的分布:免疫组织化学研究。

C Li, Y Hirooka, R Honda, R Morikawa, M Yatoh, M Gotoh, T Nogimori
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引用次数: 0

摘要

本研究的目的是利用我们研制的特异性抗肽抗血清,通过免疫组织化学方法鉴定水通道蛋白9在大鼠组织中的分布。用合成的水通道蛋白-9肽与牛血清白蛋白偶联物免疫新西兰大白兔,获得抗水通道蛋白-9抗体。免疫组化分析采用亲和素-生物素复合物法。水通道蛋白免疫反应可见于垂体前叶、中枢神经系统、视网膜、甲状腺、胃肠道、肝肺、胰腺和睾丸。使用人工合成肽预孵育的抗血清或含有水通道蛋白-9肽的大鼠下丘脑匀浆,下丘脑未见明显染色。这些结果表明水通道蛋白-9分布广泛,该方法对研究水通道蛋白-9在大鼠体内的分布有一定的价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Distribution of aquaporin-9 in the rat: an immunohistochemical study.

The aim of this study was to identify the distribution of aquaporin-9 by immunohistochemistry in rat tissues using specific antipeptide antiserum which we recently produced. Anti-aquaporin-9 antibody was raised in New Zealand white rabbits immunized with a conjugate of synthetic aquaporin-9 peptide with bovine serum albumin. Immunohistochemical analysis was performed by avidin-biotin complex method. Aquaporin-immunoreactivity was visualized in the anterior pituitary, central nervous system, retina, thyroid gland, gastrointestinal tract, liver lung, pancreas and testis. When using antiserum preincubated with synthetic peptides or rat hypothalamus homogenate, which contains aquaporin-9 peptide, no significant stain of the hypothalamus was detected. These findings suggest that aquaporin-9 is widely distributed and that the method used is valuable in studying the distribution of aquaporin-9 in rats.

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