水稻叶片和颈瘟病部分抗性的遗传分析。

Zhi-Ming Rao, Jian-Li Wu, Jie-Yun Zhuang, Rong-Yao Chai, Ye-Yang Fan, Hei Leung, Kang-Le Zheng
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引用次数: 0

摘要

在籼稻重组自交系(RIL)群体中,选择由易感系组成的2个亚群体,用2个分离株对叶瘟病菌进行部分抗性定位。利用抽穗时间相似的易感品系组成的第三个亚群,用第三个分离物绘制了对颈瘟病的部分抗性图谱。部分抗性的测定性状包括叶瘟的病叶面积(DLA)、病斑大小(LS)和病斑数(LN),颈瘟的病斑长度(LL)和分生孢子数(CA)。利用整个RIL群体构建了包含168个DNA标记的连锁图谱。在单位点和双位点水平上确定了这些性状的数量性状位点(qtl)。通过QTLMapper 1.01 b检测到11个主效qtl和28个基因相互作用。只有3个主效qtl参与了同一性状的基因相互作用。各性状上位性qtl的总贡献率为16.0% ~ 51.7%,主效qtl的总贡献率为4.7% ~ 38.8%。多数性状主效qtl的总体贡献率小于上位性qtl,证实了上位性作为复杂性状遗传基础的重要性。所有检测到的LL和CA两个性状的主效和上位效qtl的总体贡献率分别达到70.6%和82.6%,这显然是控制颈瘟病部分抗性的主要遗传基础。结果表明,在避免主要抗性基因干扰的情况下,有必要利用易感亚群进行部分抗性定位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic dissections of partial resistances to leaf and neck blast in rice (Oryza sativa L.).

In a recombinant inbred line (RIL) population of indica rice, two subpopulations composed of susceptible lines were selected for mapping of partial resistance to leaf blast with two isolates of the pathogen. A third subpopulation composed of susceptible lines with similar heading time was used for mapping of partial resistance to neck blast with a third isolate. The traits measured for partial resistance included diseased leaf area (DLA), lesion size (LS) and lesion number (LN) for leaf blast and lesion length (LL) and conidium amount (CA) for neck blast. A linkage map consisting of 168 DNA markers was constructed by using the whole RIL population. Quantitative trait loci (QTLs) conditioning these traits were determined at one-locus and two-locus levels. Eleven main-effect QTLs and 28 digenic interactions were detected by QTLMapper 1.01 b. Only three QTLs showing main effects were also involved in digenic interactions for the same trait. General contributions of epistatic QTLs of each trait ranged from 16.0% to 51.7%, while those of main-effect QTLs of each trait ranged from 4.7% to 38.8%. The general contributions of main-effect QTLs of most traits were smaller than those of epistatic QTLs, confirming the importance of epistasis as the genetic basis for complex traits. The general contributions of the main and epistatic effects of all QTLs detected for the two traits LL and CA of the partial resistance to neck blast reached 70.6% and 82.6% respectively, which obviously represented a major part of the genetic basis controlling partial resistance to neck blast. The results indicated the necessity for partial resistance mapping to use susceptible subpopulations where the interference of major resistance genes is avoided.

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