Loida V Ponce, José Corado, Nilka L Díaz, Felix J Tapia
{"title":"树突状细胞的采纳性转移可调节小鼠皮肤利什曼病新生儿模型的免疫发生和耐受发生。","authors":"Loida V Ponce, José Corado, Nilka L Díaz, Felix J Tapia","doi":"10.1186/1475-9292-4-2","DOIUrl":null,"url":null,"abstract":"<p><p>We evaluated the adoptive transfer of DCs on <i>Leishmania (L.) mexicana</i>-infected neonatal BALB/c mice. DCs were isolated and purified from the spleens of the following donor groups: a) Adult BALB/c mice infected during adulthood with <i>L. (L) mexicana</i>; b) Adult BALB/c mice infected during neonatal life; c) Healthy neonatal BALB/c mice; d) Healthy adult BALB/c mice. A neonatal model of infection, generated after inoculation with 5 × 10<sup>5</sup> promastigotes of <i>L. (L) mexicana</i>, was used as the infection control group. Sixteen hours after intraperitoneal transfer of DCs (1 × 10<sup>3</sup>, 1 × 10<sup>5</sup>, or 1 × 10<sup>6</sup> cells/ml), neonatal recipient BALB/c mice were infected. The adoptive transfer of DCs diminished disease progression in neonatal mice. This reduction depends on the quantity and provenance of transferred DCs, since the effect was more evident with high numbers of DCs from adult mice infected during adulthood and healthy neonatal mice. Protection was significantly reduced in animals receiving DCs from healthy adult mice but it was absent in mice receiving DCs from adult mice infected during neonatal life. These results suggest that genetic susceptibility to <i>Leishmania</i> infection can be modified during neonatal life, and that the period of life when antigens are encountered is crucial in influencing the capacity of DCs to induce resistance or tolerance.</p>","PeriodicalId":17853,"journal":{"name":"Kinetoplastid Biology and Disease","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548294/pdf/","citationCount":"0","resultStr":"{\"title\":\"Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.\",\"authors\":\"Loida V Ponce, José Corado, Nilka L Díaz, Felix J Tapia\",\"doi\":\"10.1186/1475-9292-4-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We evaluated the adoptive transfer of DCs on <i>Leishmania (L.) mexicana</i>-infected neonatal BALB/c mice. DCs were isolated and purified from the spleens of the following donor groups: a) Adult BALB/c mice infected during adulthood with <i>L. (L) mexicana</i>; b) Adult BALB/c mice infected during neonatal life; c) Healthy neonatal BALB/c mice; d) Healthy adult BALB/c mice. A neonatal model of infection, generated after inoculation with 5 × 10<sup>5</sup> promastigotes of <i>L. (L) mexicana</i>, was used as the infection control group. Sixteen hours after intraperitoneal transfer of DCs (1 × 10<sup>3</sup>, 1 × 10<sup>5</sup>, or 1 × 10<sup>6</sup> cells/ml), neonatal recipient BALB/c mice were infected. The adoptive transfer of DCs diminished disease progression in neonatal mice. This reduction depends on the quantity and provenance of transferred DCs, since the effect was more evident with high numbers of DCs from adult mice infected during adulthood and healthy neonatal mice. Protection was significantly reduced in animals receiving DCs from healthy adult mice but it was absent in mice receiving DCs from adult mice infected during neonatal life. These results suggest that genetic susceptibility to <i>Leishmania</i> infection can be modified during neonatal life, and that the period of life when antigens are encountered is crucial in influencing the capacity of DCs to induce resistance or tolerance.</p>\",\"PeriodicalId\":17853,\"journal\":{\"name\":\"Kinetoplastid Biology and Disease\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-01-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548294/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Kinetoplastid Biology and Disease\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/1475-9292-4-2\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2005/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kinetoplastid Biology and Disease","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/1475-9292-4-2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2005/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
我们对利什曼原虫(L. mexicana)感染新生 BALB/c 小鼠的 DCs 领养转移进行了评估。我们从以下供体组小鼠的脾脏中分离并纯化了 DCs:a) 成年期感染墨西哥利什曼原虫的成年 BALB/c 小鼠;b) 新生儿期感染墨西哥利什曼原虫的成年 BALB/c 小鼠;c) 健康的新生 BALB/c 小鼠;d) 健康的成年 BALB/c 小鼠。新生儿感染模型是在接种了 5 × 105 个 L. (L) mexicana 原虫后产生的,作为感染对照组。腹腔转移 DC(1 × 103、1 × 105 或 1 × 106 cells/ml)16 小时后,新生受体 BALB/c 小鼠受到感染。DCs的收养性转移减轻了新生小鼠的疾病进展。这种缓解作用取决于转移的 DC 数量和来源,因为来自成年期感染的成年小鼠和健康新生小鼠的 DC 数量多时,效果更明显。接受来自健康成年小鼠的 DC 的动物的保护作用明显降低,但接受来自在新生儿期感染的成年小鼠的 DC 的小鼠则没有保护作用。这些结果表明,对利什曼原虫感染的遗传易感性可在新生儿期发生改变,而生命中遇到抗原的时期对于影响直流细胞诱导抵抗或耐受的能力至关重要。
Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.
We evaluated the adoptive transfer of DCs on Leishmania (L.) mexicana-infected neonatal BALB/c mice. DCs were isolated and purified from the spleens of the following donor groups: a) Adult BALB/c mice infected during adulthood with L. (L) mexicana; b) Adult BALB/c mice infected during neonatal life; c) Healthy neonatal BALB/c mice; d) Healthy adult BALB/c mice. A neonatal model of infection, generated after inoculation with 5 × 105 promastigotes of L. (L) mexicana, was used as the infection control group. Sixteen hours after intraperitoneal transfer of DCs (1 × 103, 1 × 105, or 1 × 106 cells/ml), neonatal recipient BALB/c mice were infected. The adoptive transfer of DCs diminished disease progression in neonatal mice. This reduction depends on the quantity and provenance of transferred DCs, since the effect was more evident with high numbers of DCs from adult mice infected during adulthood and healthy neonatal mice. Protection was significantly reduced in animals receiving DCs from healthy adult mice but it was absent in mice receiving DCs from adult mice infected during neonatal life. These results suggest that genetic susceptibility to Leishmania infection can be modified during neonatal life, and that the period of life when antigens are encountered is crucial in influencing the capacity of DCs to induce resistance or tolerance.