{"title":"新贻贝hsp70基因转录单位的分析与表征。","authors":"Antonis Kourtidis, Zacharias G Scouras","doi":"10.1080/10425170400024391","DOIUrl":null,"url":null,"abstract":"<p><p>We isolated, analysed and characterized the transcriptional unit of an hsp70 gene of Mytilus galloprovincialis (Mollusca: Bivalvia). Sequence analysis of a total of 3653 bp, revealed a 1914 bp ORF encoding for a 637-amino acid polypeptide that exhibits all the characteristic signatures of the cytoplasmic members of the HSP70 family. The ORF is not interrupted by introns and its 5'-flanking region contains five putative heat shock elements (HSEs), both indicating for an inducible hsp70 gene. Furthermore, nucleotide and amino acid sequence alignments with HSP70 genes from several molluscan and non-molluscan species confirmed that the newly isolated gene, the first complete in the genus Mytilus and in the Mollusca phylum, is a cytoplasmic and inducible hsp70 gene.</p>","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"16 1","pages":"36-43"},"PeriodicalIF":0.0000,"publicationDate":"2005-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170400024391","citationCount":"12","resultStr":"{\"title\":\"Analysis and characterization of the transcriptional unit of a new Mytilus galloprovincialis (Mollusca: Bivalvia) hsp70 gene.\",\"authors\":\"Antonis Kourtidis, Zacharias G Scouras\",\"doi\":\"10.1080/10425170400024391\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We isolated, analysed and characterized the transcriptional unit of an hsp70 gene of Mytilus galloprovincialis (Mollusca: Bivalvia). Sequence analysis of a total of 3653 bp, revealed a 1914 bp ORF encoding for a 637-amino acid polypeptide that exhibits all the characteristic signatures of the cytoplasmic members of the HSP70 family. The ORF is not interrupted by introns and its 5'-flanking region contains five putative heat shock elements (HSEs), both indicating for an inducible hsp70 gene. Furthermore, nucleotide and amino acid sequence alignments with HSP70 genes from several molluscan and non-molluscan species confirmed that the newly isolated gene, the first complete in the genus Mytilus and in the Mollusca phylum, is a cytoplasmic and inducible hsp70 gene.</p>\",\"PeriodicalId\":11197,\"journal\":{\"name\":\"DNA sequence : the journal of DNA sequencing and mapping\",\"volume\":\"16 1\",\"pages\":\"36-43\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/10425170400024391\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"DNA sequence : the journal of DNA sequencing and mapping\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10425170400024391\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA sequence : the journal of DNA sequencing and mapping","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10425170400024391","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Analysis and characterization of the transcriptional unit of a new Mytilus galloprovincialis (Mollusca: Bivalvia) hsp70 gene.
We isolated, analysed and characterized the transcriptional unit of an hsp70 gene of Mytilus galloprovincialis (Mollusca: Bivalvia). Sequence analysis of a total of 3653 bp, revealed a 1914 bp ORF encoding for a 637-amino acid polypeptide that exhibits all the characteristic signatures of the cytoplasmic members of the HSP70 family. The ORF is not interrupted by introns and its 5'-flanking region contains five putative heat shock elements (HSEs), both indicating for an inducible hsp70 gene. Furthermore, nucleotide and amino acid sequence alignments with HSP70 genes from several molluscan and non-molluscan species confirmed that the newly isolated gene, the first complete in the genus Mytilus and in the Mollusca phylum, is a cytoplasmic and inducible hsp70 gene.
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