T-DNA转运与整合的分子机制研究进展。

Ya-Guang Zhan, Fan-Suo Zeng, Ying Xin
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摘要

农杆菌介导的转化可能是最广泛使用的将基因导入植物的方法。近年来,对农杆菌介导的转化机制的研究取得了很大进展。农杆菌通过将一部分ti质粒(T-DNA)转移到植物中,从而在基因上改变植物。VirD2和VirE2随T-DNA进入植物细胞。这两种蛋白可能有助于T-DNA转移、核靶向和整合到植物基因组中。近年来,一些研究发现一些拟南芥大鼠(抗转化)突变体存在T-DNA整合缺陷。这些结果表明,植物基因参与了T-DNA的转运和整合过程。本文讨论了目前我们所知的毒力蛋白VirD2和VirE2以及植物基因在农杆菌转化过程中几个方面的功能。本文详细讨论了两种不同类型的整合模式:一种是通过VirD2蛋白连接到基因组序列的T-DNA右边界,另一种是通过SDSA(合成依赖链退火)进行的T-DNA整合。根据最新的研究成果,我们阐述了基于基因组双链断裂的T-DNA整合模型,并对T-DNA整合的机制提出了新的看法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Progress on molecular mechanism of T-DNA transport and integration.

Agrobacterium-mediated transformation is probably the most widely used method to introduce genes into plants. Great progress has been made in recent years in studies on the mechanism of Agrobacterium-mediated transformation. Agrobacterium genetically transforms plants by transferring a portion of the resident Ti-plasmid, the T-DNA, to the plant. VirD2 and VirE2 accompany the T-DNA into the plant cell. Both proteins may aid in T-DNA transfer, nuclear targeting and integration into the plant genome. In recent years, some Arabidopsis rat (resistant to transformation) mutants are deficient in T-DNA integration according to some studies. These results showed that plant genes participate in the T-DNA transport and integration process. This paper discusses our current knowledge about the functions of virulence protein, namely VirD2 and VirE2, and plant genes in several aspect of Agrobacterium transformation. The paper discusses two different classes of integration patterns in detail: one is T-DNA right border being linked to genomic sequences by the VirD2 protein, the other is T-DNA integration via SDSA (synthesis-dependent strand-annealing). According to the latest studies we elaborated the T-DNA integration model based on genomic DSB (double-strand breaks) and proposed a new opinion about the mechanism of T-DNA integration.

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