Miriane da Costa, Valdecir Farias Ximenes, Iguatemy Lourenço Brunetti, Roberto Passetto Falcão, Luiz Marcos da Fonseca
{"title":"应用对碘酚增强发光胺化学发光法鉴别急性淋巴母细胞白血病和轻度分化急性髓细胞白血病(FAB-M0)或急性巨核母细胞白血病(FAB-M7)。","authors":"Miriane da Costa, Valdecir Farias Ximenes, Iguatemy Lourenço Brunetti, Roberto Passetto Falcão, Luiz Marcos da Fonseca","doi":"10.1038/sj.thj.6200562","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).</p><p><strong>Methods: </strong>The frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.</p><p><strong>Results: </strong>All cases of AML-M7 and AML-M I exhibited integrated light emission greater than 73(10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-MO showed results above the cutoff point.</p><p><strong>Conclusion: </strong>This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-MI cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.</p>","PeriodicalId":22486,"journal":{"name":"The hematology journal : the official journal of the European Haematology Association","volume":"5 6","pages":"496-9"},"PeriodicalIF":0.0000,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"p-Iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemias.\",\"authors\":\"Miriane da Costa, Valdecir Farias Ximenes, Iguatemy Lourenço Brunetti, Roberto Passetto Falcão, Luiz Marcos da Fonseca\",\"doi\":\"10.1038/sj.thj.6200562\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).</p><p><strong>Methods: </strong>The frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.</p><p><strong>Results: </strong>All cases of AML-M7 and AML-M I exhibited integrated light emission greater than 73(10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-MO showed results above the cutoff point.</p><p><strong>Conclusion: </strong>This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-MI cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.</p>\",\"PeriodicalId\":22486,\"journal\":{\"name\":\"The hematology journal : the official journal of the European Haematology Association\",\"volume\":\"5 6\",\"pages\":\"496-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2004-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The hematology journal : the official journal of the European Haematology Association\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1038/sj.thj.6200562\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The hematology journal : the official journal of the European Haematology Association","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1038/sj.thj.6200562","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
在这篇报道中，我们提出应用对碘酚增强发光胺化学发光技术来测定最小分化急性髓母细胞白血病(AML-M0)和急性巨核母细胞白血病(AML-M7)母细胞的过氧化物酶(髓过氧化物酶和/或血小板过氧化物酶)活性。方法:对29例患者的冷冻胚细胞进行解冻处理。结果:AML- m7和AML- m - I的综合光发射均大于73(10(2)mV x s)，这是区分AML和急性淋巴细胞白血病(All)的任意截断点(All样本的平均值+ 3 x sd, n = 10)。此外，7例AML-MO中有5例的结果高于临界值。结论:这种高灵敏度的增强化学发光技术可用于ALL和AML-M7或AML-MI病例以及大多数AML-M0病例的鉴别。与用于测量AML-M7和AML-M0等aml -白血病中MPO活性的其他程序相比，该方法非常简单，便宜且易于执行。
p-Iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemias.
Introduction: In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).
Methods: The frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.
Results: All cases of AML-M7 and AML-M I exhibited integrated light emission greater than 73(10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-MO showed results above the cutoff point.
Conclusion: This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-MI cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
