N Doménech, B Alvarez, R Bullido, F Alonso, A Ezquerra, J Domínguez
{"title":"单克隆抗体5F12/9在猪CD5分子上检测到一个新的表位。","authors":"N Doménech, B Alvarez, R Bullido, F Alonso, A Ezquerra, J Domínguez","doi":"10.1089/153685903322286601","DOIUrl":null,"url":null,"abstract":"<p><p>This paper describes the production and characterization of a monoclonal antibody (MAb), 5F12/9, that recognizes a new epitope on porcine CD5. Conformation of its CD5 specificity was obtained by means of sequential immunoprecipitation and Western blot experiments in combination with anti-CD5 MAb 1H6/8, whereas cross-blocking experiments with both MAbs showed that they reacted with different epitopes.</p>","PeriodicalId":83733,"journal":{"name":"Hybridoma and hybridomics","volume":"22 3","pages":"179-82"},"PeriodicalIF":0.0000,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/153685903322286601","citationCount":"1","resultStr":"{\"title\":\"A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.\",\"authors\":\"N Doménech, B Alvarez, R Bullido, F Alonso, A Ezquerra, J Domínguez\",\"doi\":\"10.1089/153685903322286601\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This paper describes the production and characterization of a monoclonal antibody (MAb), 5F12/9, that recognizes a new epitope on porcine CD5. Conformation of its CD5 specificity was obtained by means of sequential immunoprecipitation and Western blot experiments in combination with anti-CD5 MAb 1H6/8, whereas cross-blocking experiments with both MAbs showed that they reacted with different epitopes.</p>\",\"PeriodicalId\":83733,\"journal\":{\"name\":\"Hybridoma and hybridomics\",\"volume\":\"22 3\",\"pages\":\"179-82\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1089/153685903322286601\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hybridoma and hybridomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/153685903322286601\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hybridoma and hybridomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/153685903322286601","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9.
This paper describes the production and characterization of a monoclonal antibody (MAb), 5F12/9, that recognizes a new epitope on porcine CD5. Conformation of its CD5 specificity was obtained by means of sequential immunoprecipitation and Western blot experiments in combination with anti-CD5 MAb 1H6/8, whereas cross-blocking experiments with both MAbs showed that they reacted with different epitopes.