Karine Faure, Junichi Fujimoto, David W Shimabukuro, Temitayo Ajayi, Nobuaki Shime, Kiyoshi Moriyama, Edward G Spack, Jeanine P Wiener-Kronish, Teiji Sawa
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引用次数: 74
摘要
背景:在大鼠模型上分析小鼠抗pcrv单克隆抗体Mab166对铜绿假单胞菌急性肺损伤的作用。方法:将铜绿假单胞菌PA103直接滴入麻醉大鼠左肺诱导肺损伤。细菌滴注1小时后，兔多克隆抗pcrv IgG、鼠单克隆抗pcrv IgG Mab166或Mab166 fab片段经气管直接注入肺内。肺泡上皮损伤程度、肺水肿量、肺氧合减少和肺炎症程度作为急性肺损伤的独立参数进行组织学评价。结果:气管内注射兔多克隆抗pcrv IgG、鼠单克隆抗pcrv IgG Mab166或Mab166 fab -片段的大鼠与对照组相比，这些参数有所改善。结论:Mab166及其Fab片段具有辅助治疗铜绿假单胞菌肺炎急性肺损伤的潜力。
Effects of monoclonal anti-PcrV antibody on Pseudomonas aeruginosa-induced acute lung injury in a rat model.
BACKGROUND: The effects of the murine monoclonal anti-PcrV antibody Mab166 on acute lung injury induced by Pseudomonas aeruginosa were analyzed in a rat model. METHODS: Lung injury was induced by the instillation of P. aeruginosa strain PA103 directly into the left lungs of anesthetized rats. One hour after the bacterial instillation, rabbit polyclonal anti-PcrV IgG, murine monoclonal anti-PcrV IgG Mab166 or Mab166 Fab-fragments were administered intratracheally directly into the lungs. The degree of alveolar epithelial injury, amount of lung edema, decrease in oxygenation and extent of lung inflammation by histology were evaluated as independent parameters of acute lung injury. RESULTS: These parameters improved in rats that had received intratracheal instillation of either rabbit polyclonal anti-PcrV IgG, murine monoclonal anti-PcrV IgG Mab166 or Mab166 Fab-fragments in comparison with the control group. CONCLUSION: Mab166 and its Fab fragments have potential as adjuvant therapy for acute lung injury due to P. aeruginosa pneumonia.
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