组蛋白H1对长双工DNA的盐依赖性折叠。

Y Yoshikawa, T Kanbe
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引用次数: 0

摘要

在高盐溶液(2 M NaCl)和低盐溶液(50 mM NaCl)中进行温和稀释后,发现T4噬菌体DNA与组蛋白H1络合物组装成一串珠状结构。我们使用荧光显微镜对串珠结构的形成和运动进行实时观察。免疫荧光显微镜观察组蛋白H1在DNA-H1复合体上的空间分布。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Salt-dependent folding of long duplex DNA by histone H1.

It is found that T4 phage DNA complexed with histone H1 assembled into a string-of-bead structure, when the complex is prepared by a gentle diluting procedure from a high salt solution (2 M NaCl) to a low salt solution (50 mM NaCl). We used fluorescence microscopy to perform the real-time observation on formation and motion of a string-of-bead structure. Spatial histone H1 distribution on the DNA-H1 complex is observed by immuno-fluorescence microscopy.

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