哺乳动物tRNA 3'加工核糖核酸内切酶和外部引导序列寡酶有效抑制HIV-1基因表达

M Kitano, N Miyano-Kurosaki, Y Endo, M Yukita, H Takeuchi, Y Tamura, K Takai, M Nashimoto, H Takaku
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引用次数: 0

摘要

我们使用哺乳动物tRNA 3'加工核糖核酸内切酶和外部引导序列寡聚酶(EGS)在体内检测了HIV-1 RNA基因切割对病毒表达的抑制作用。我们构建了一个EGS表达载体,使用tRNA(met)启动子作为EGS的表达盒。EGS表达载体定位于gag区上游区域。将EGS表达载体与HIV-1基因质粒载体共转染COS细胞。与EGS非表达细胞和EGS表达细胞相比,靶向gag启动密码子的EGS表达细胞的HIV-1 gag p24蛋白含量明显降低。具有靶向gag启动密码子的EGS表达细胞能有效抑制HIV-1基因的表达。因此,这些研究描述了抑制基因表达和抗病毒活性的新型基因靶向剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effective suppression of HIV-1 gene expression by a mammalian tRNA 3' processing endoribonuclease and external guide sequence oligozymes.

We examined the suppression of virus expression by cleavage of the HIV-1 RNA gene using a mammalian tRNA 3' processing endoribonuclease and an External Guide Sequence Oligozyme (EGS) in vivo. We constructed an EGS expression vector that used the tRNA(met) promoter as an expression cassette for EGS. The EGS expression vector was targeted to the upstream region of gag, region. The EGS expression vector was co-transfected into COS cells with the HIV-1 gene plasmid vector. As compared with the EGS non-expressing cells and the EGS expressing cells, the EGS expressing cells with the targeted gag start codon had a clearly decreased amount of the HIV-1 gag p24 protein. The EGS expressing cells with the targeted gag start codon showed effective suppression of HIV-1 gene expression. Thus, these studies describe novel gene targeting agents for the inhibition of gene expression and antiviral activity.

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