利用突变GCN4-bZIP肽进行体外筛选，分析肽与dna的相互作用。

Nucleic acids symposium series Pub Date : 2000-01-01 DOI:10.1093/nass/44.1.245

H Furusawa, T Morii, Y Okahata

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引用次数: 1

摘要

体外选择已被用作确定dna结合蛋白最佳结合位点的方法。我们在这里报道了结合突变gcn4 - bzip肽的dsDNA序列的体外选择。GCN4-bZIP肽在与野生型肽结合位点不同序列的DNA接触的位置14上从丙氨酸突变为组氨酸。

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In vitro selection by using mutated GCN4-bZIP peptides for analysis of peptide-DNA interactions.

In vitro selection has been used as a method to determine the optimal binding site for DNA-binding proteins. We report here in vitro selection of dsDNA sequences that bind to mutated-GCN4-bZIP peptides. The GCN4-bZIP peptide mutated from alanine to histidine on a position-14 that contacts with DNA bound to different sequence from a binding site of wild type peptide.

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Nucleic acids symposium series

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