同嘌呤寡核苷酸形成三络合物的热力学分析。

H Torigoe, R Shimizume
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引用次数: 1

摘要

用等温滴定量热法分析了嘌呤基序三联体形成的热力学。在15 ~ 35℃的温度范围内,三相体系的热焓变δ Hcal和熵变δ S均为负,负δ S不利于三相体系的形成,因此三相体系的形成是由一个较大的负δ Hcal驱动的。δ Hcal随温度升高而降低,产生负的热容量变化δ Cp,约为-1.2 kcal mol-1 K-1。我们发现,结合常数Ka随着温度的升高而增加,导致明显的正范霍夫焓变δ Hvh,这与较大的负δ Hcal形成鲜明对比。观察到的Ka和δ Hcal的温度依赖性和负δ Cp的分析表明,室温附近嘌呤基序三联体的形成不是一个简单的两态结合过程,而是呈现出多态的结合过程,这与之前在室温附近嘧啶基序三联体的形成是一致的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Thermodynamic analyses of triplex formation with homopurine oligonucleotide.

We analyzed the thermodynamics of purine motif triplex formation by isothermal titration calorimetry. The signs of calorimetric enthalpy change, delta Hcal, and entropy change, delta S, of the triplex formation were negative in the temperature range between 15 and 35 degrees C. Since an observed negative delta S was unfavorable for the triplex formation, the triplex formation was driven by a large negative delta Hcal. delta Hcal decreased with increasing temperature, yielding a negative heat capacity change, delta Cp, of approximately -1.2 kcal mol-1 K-1. We found that the binding constant, Ka, increased with increasing temperature, leading to an apparent positive van't Hoff enthalpy change, delta Hvh, which was in sharp contrast with the large negative delta Hcal. The analyses of the observed temperature dependence of Ka and delta Hcal and the negative delta Cp suggest that the purine motif triplex formation near room temperature is not a simple two-state binding process but exhibits multiple states, which was previously observed for the pyrimidine motif triplex formation near room temperature.

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