{"title":"巨噬细胞集落刺激因子和白细胞介素-2对小鼠NK1.1+细胞的影响","authors":"Eriko Misawa , Takuma Sakurai , Muneo Yamada , Hirotoshi Hayasawa , Kazuo Motoyoshi","doi":"10.1016/S0192-0561(00)00061-8","DOIUrl":null,"url":null,"abstract":"<div><p>We studied the effects of M-CSF and IL-2 on NK1.1<sup>+</sup> cell activity in vivo and in vitro. Administration of M-CSF increased the number of splenic NK1.1<sup>+</sup> cells (vs. saline: <em>P</em><0.01). Moreover, the combination of M-CSF and IL-2 (M-CSF+IL-2) produced a synergistic expansion of the number of NK1.1<sup>+</sup> cells compared with each single treatment (vs. saline: <em>P</em><0.001). The NK1.1<sup>+</sup> cells were isolated from the spleen of each treated mouse (four treatment groups: saline, IL-2 alone, M-CSF alone, M-CSF+IL-2) and their functions (IL-2-induced proliferation, IFN-<em>γ</em> production and cytostatic activity) were evaluated in vitro. The NK1.1<sup>+</sup> cells from M-CSF alone and M-CSF+IL-2 treated mice showed greater responsiveness in terms of IL-2-induced proliferation, production of IFN-<em>γ</em><span><span> and cytostatic activity<span> than the cells from saline and IL-2 alone treated mice. The NK activity in vivo was enhanced by the administration of M-CSF and IL-2, as assessed by the ‘Lung clearance assay’ (clearance of Yac-1 cells in lung). And the M-CSF+IL-2 treatment induced the highest NK activity of the four treatments. To show a practical effect of upregulation of NK activity in vivo by M-CSF and IL-2 administration, the effect of the four treatments on an experimental tumor metastasis<span> model was examined. The IL-2 alone, M-CSF alone and M-CSF+IL-2 treatment reduced the metastasis of B16 melanoma. And the M-CSF+IL-2 treatment proved of greater benefit to the </span></span></span>antimetastatic activity than each single treatment. Our results demonstrated that the administration of M-CSF increases the number of NK1.1</span><sup>+</sup> cells, which have good responsiveness to IL-2. Furthermore, the combination treatment of M-CSF and IL-2 in vivo augments the increase of NK1.1<sup>+</sup> cells. And these effects can contribute to the antimetastatic activity in vivo.</p></div>","PeriodicalId":14002,"journal":{"name":"International journal of immunopharmacology","volume":"22 11","pages":"Pages 967-977"},"PeriodicalIF":0.0000,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00061-8","citationCount":"24","resultStr":"{\"title\":\"Effects of macrophage colony-stimulating factor and interleukin-2 administration on NK1.1+ cells in mice\",\"authors\":\"Eriko Misawa , Takuma Sakurai , Muneo Yamada , Hirotoshi Hayasawa , Kazuo Motoyoshi\",\"doi\":\"10.1016/S0192-0561(00)00061-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We studied the effects of M-CSF and IL-2 on NK1.1<sup>+</sup> cell activity in vivo and in vitro. Administration of M-CSF increased the number of splenic NK1.1<sup>+</sup> cells (vs. saline: <em>P</em><0.01). Moreover, the combination of M-CSF and IL-2 (M-CSF+IL-2) produced a synergistic expansion of the number of NK1.1<sup>+</sup> cells compared with each single treatment (vs. saline: <em>P</em><0.001). The NK1.1<sup>+</sup> cells were isolated from the spleen of each treated mouse (four treatment groups: saline, IL-2 alone, M-CSF alone, M-CSF+IL-2) and their functions (IL-2-induced proliferation, IFN-<em>γ</em> production and cytostatic activity) were evaluated in vitro. The NK1.1<sup>+</sup> cells from M-CSF alone and M-CSF+IL-2 treated mice showed greater responsiveness in terms of IL-2-induced proliferation, production of IFN-<em>γ</em><span><span> and cytostatic activity<span> than the cells from saline and IL-2 alone treated mice. The NK activity in vivo was enhanced by the administration of M-CSF and IL-2, as assessed by the ‘Lung clearance assay’ (clearance of Yac-1 cells in lung). And the M-CSF+IL-2 treatment induced the highest NK activity of the four treatments. To show a practical effect of upregulation of NK activity in vivo by M-CSF and IL-2 administration, the effect of the four treatments on an experimental tumor metastasis<span> model was examined. The IL-2 alone, M-CSF alone and M-CSF+IL-2 treatment reduced the metastasis of B16 melanoma. And the M-CSF+IL-2 treatment proved of greater benefit to the </span></span></span>antimetastatic activity than each single treatment. Our results demonstrated that the administration of M-CSF increases the number of NK1.1</span><sup>+</sup> cells, which have good responsiveness to IL-2. Furthermore, the combination treatment of M-CSF and IL-2 in vivo augments the increase of NK1.1<sup>+</sup> cells. And these effects can contribute to the antimetastatic activity in vivo.</p></div>\",\"PeriodicalId\":14002,\"journal\":{\"name\":\"International journal of immunopharmacology\",\"volume\":\"22 11\",\"pages\":\"Pages 967-977\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0192-0561(00)00061-8\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of immunopharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0192056100000618\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of immunopharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0192056100000618","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effects of macrophage colony-stimulating factor and interleukin-2 administration on NK1.1+ cells in mice
We studied the effects of M-CSF and IL-2 on NK1.1+ cell activity in vivo and in vitro. Administration of M-CSF increased the number of splenic NK1.1+ cells (vs. saline: P<0.01). Moreover, the combination of M-CSF and IL-2 (M-CSF+IL-2) produced a synergistic expansion of the number of NK1.1+ cells compared with each single treatment (vs. saline: P<0.001). The NK1.1+ cells were isolated from the spleen of each treated mouse (four treatment groups: saline, IL-2 alone, M-CSF alone, M-CSF+IL-2) and their functions (IL-2-induced proliferation, IFN-γ production and cytostatic activity) were evaluated in vitro. The NK1.1+ cells from M-CSF alone and M-CSF+IL-2 treated mice showed greater responsiveness in terms of IL-2-induced proliferation, production of IFN-γ and cytostatic activity than the cells from saline and IL-2 alone treated mice. The NK activity in vivo was enhanced by the administration of M-CSF and IL-2, as assessed by the ‘Lung clearance assay’ (clearance of Yac-1 cells in lung). And the M-CSF+IL-2 treatment induced the highest NK activity of the four treatments. To show a practical effect of upregulation of NK activity in vivo by M-CSF and IL-2 administration, the effect of the four treatments on an experimental tumor metastasis model was examined. The IL-2 alone, M-CSF alone and M-CSF+IL-2 treatment reduced the metastasis of B16 melanoma. And the M-CSF+IL-2 treatment proved of greater benefit to the antimetastatic activity than each single treatment. Our results demonstrated that the administration of M-CSF increases the number of NK1.1+ cells, which have good responsiveness to IL-2. Furthermore, the combination treatment of M-CSF and IL-2 in vivo augments the increase of NK1.1+ cells. And these effects can contribute to the antimetastatic activity in vivo.