水稻幼苗植物螯合素合成酶的分离与鉴定。

S L Yan, C C Tsay, Y R Chen
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引用次数: 0

摘要

用50微米硫酸铜处理水稻植株,通过光螯合素合成酶等一系列酶促反应诱导植物螯合素的合成。采用冰冻丙酮沉淀、QAE a -50阴离子交换柱、Amicon XM-50超滤、Polybuffer exchange (PBE) 94色谱聚焦等步骤纯化3周龄水稻幼苗中的植物螯合素合成酶。该酶的分子量约为100 kDa,等电点为4.0。该酶的最适温度为55℃,最适pH为7.5。该酶耐热,在4℃或-20℃的冷藏条件下不稳定。镉是最有效的刺激剂,其次是铅、铜、银、钴和其他二价阳离子。钙和镁则没有效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of phytochelatin synthase in rice seedlings.

Rice plants were treated with 50 microM copper sulfate to induce the synthesis of phytochelatins by means of a series of enzymatic reactions, including that of photochelatin synthase. Phytochelatin synthase extracted from 3-week-old rice seedlings was purified through a series of steps including precipitation with ice-chilled acetone, QAE A-50 anion exchange column, Amicon XM-50 ultrafiltration and Polybuffer Exchange (PBE) 94 chromato-focusing. This enzyme had a molecular mass of about 100 kDa with an isoelectric point of 4.0. The temperature and pH optima of this enzyme were 55 degrees C and pH 7.5, respectively. The enzyme was thermal tolerable and unstable under refrigeration at 4 or -20 degrees C. Cadmium was the most effective stimulator, followed by lead, copper, silver, cobalt and other divalent cations. Calcium and magnesium had no effect.

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