阿特拉津、硫丹和丁基羟基茴香醚对枸杞谷胱甘肽- s转移酶的影响

Eliann Egaas
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引用次数: 5

摘要

在半合成日粮中添加阿特拉嗪(1000 ppm)、硫丹(1 ppm)或丁基羟基茴香醚(1000 ppm),对1-氯-2,4-二硝基苯(CDNB)、1,2-二氯-4-硝基苯(DCNB)和异丙苯氢过氧化物(CU)的软组织胞浆谷胱甘肽-s -转移酶(GST)活性没有影响。然而,通过谷胱甘肽- sepharose亲和层析纯化的同工酶的反相高效液相色谱观察到,与对照相比,这三种农药都改变了GST亚基组成。这些变化似乎主要发生在商品及服务税第2类亚单位。这种变化没有明显的解释,这可能是细胞质中异种生物和GST相互作用以及合成调节水平变化的结果。然而,这一观察增加了我们对农药在体内被gst降解所涉及的过程的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of atrazine, endosulfan and butylated hydroxyanisole on glutathione-S-transferases in Orthosia Gothica

Atrazine (1000 ppm), endosulfan (1 ppm) or butylated hydroxyanisole (BHA) (1000 ppm) added to a semi-synthetic diet of Orthosia gothica for 2 days in the last instar did not change the soft tissue cytosolic glutathione-S-transferase (GST) activities towards 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB) and cumene hydroperoxide (CU). However, all three pesticides changed the GST subunit composition compared with the control as observed by reverse phase high performance liquid chromatography of the isozymes purified by glutathione–Sepharose affinity chromatography. The changes seem to have occurred mainly in the GST class 2 subunit. There is no obvious explanation for the changes, which may be a result of interactions between xenobiotic and GST in the cytosol as well as changes in the level of regulation of synthesis. However, the observation added to our knowledge of the processes involved when pesticides are degraded by GSTs in vivo.

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