脂质双层结构

John F. Nagle , Stephanie Tristram-Nagle
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引用次数: 2199

摘要

完全水合的、生物相关的流体(Lα)相脂质双层结构的定量实验不确定性太大,无法为应用或与模拟比较提供坚实的基础。本文回顾了许多结构方法,包括处理在Lα相中发生的充分发展的波动的脂质双层的现代液晶学。这些波动在某种程度上降低了高阶衍射数据,如果不被识别,就会导致有关双层结构的错误结论。高仪器分辨率的衍射测量提供了对这些波动的测量。除了提供更好的结构测定外,这为双层相互作用的研究打开了一个新的窗口,因此对双层相互作用参数的实验测定进行了简要的回顾。我们引入了一种新的基于波动的结构修正,这在以前的任何研究中都没有包括在内。更新的测量,如面积压缩模量,用于对许多结构量的文献值进行调整。由于凝胶(Lβ′)相是获得流体相结果的有价值的垫脚石,因此简要回顾了低温相。脂质双分子层结构结果的不确定性正在降低,并为五种脂质双分子层提供了最佳电流值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structure of lipid bilayers

The quantitative experimental uncertainty in the structure of fully hydrated, biologically relevant, fluid (Lα) phase lipid bilayers has been too large to provide a firm base for applications or for comparison with simulations. Many structural methods are reviewed including modern liquid crystallography of lipid bilayers that deals with the fully developed undulation fluctuations that occur in the Lα phase. These fluctuations degrade the higher order diffraction data in a way that, if unrecognized, leads to erroneous conclusions regarding bilayer structure. Diffraction measurements at high instrumental resolution provide a measure of these fluctuations. In addition to providing better structural determination, this opens a new window on interactions between bilayers, so the experimental determination of interbilayer interaction parameters is reviewed briefly. We introduce a new structural correction based on fluctuations that has not been included in any previous studies. Updated measurements, such as for the area compressibility modulus, are used to provide adjustments to many of the literature values of structural quantities. Since the gel (Lβ′) phase is valuable as a stepping stone for obtaining fluid phase results, a brief review is given of the lower temperature phases. The uncertainty in structural results for lipid bilayers is being reduced and best current values are provided for bilayers of five lipids.

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