T A Wyatt, S C Schmidt, S I Rennard, D J Tuma, J H Sisson
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引用次数: 0
摘要
此前,我们发现香烟烟雾中的挥发性成分乙醛会刺激蛋白激酶 C (PKC) 通路并抑制纤毛运动。现在有一种 "无烟 "香烟(Eclipse),其中大部分烟草没有燃烧,从而减少了提取物中的热解成分。我们假设乙醛是香烟烟雾中能激活气道上皮细胞 PKC 的成分,因此 Eclipse 香烟不会激活上皮细胞 PKC。在这项研究中,牛支气管上皮细胞(BBEC)与香烟烟雾提取物(CSE)或日食烟雾提取物(ESE)一起培养。我们发现,暴露于 5% CSE 的细胞的 PKC 活性明显高于暴露于 5% ESE 或培养基的细胞。当用气相色谱法测量两种提取物的乙醛含量时,发现 CSE 的乙醛浓度(微摩尔)是 ESE 的 15-20 倍。用 5% CSE 处理 BBEC 时,睫状肌跳动在基线水平的基础上进一步减少。这种纤毛跳动的减少在用 ESE 处理的细胞中没有观察到,这表明 CSE 中含有的乙醛会减慢纤毛的跳动。这些结果表明,香烟烟雾中的乙醛等挥发性成分可能会通过 PKC 依赖性机制抑制纤毛运动。
Acetaldehyde-stimulated PKC activity in airway epithelial cells treated with smoke extract from normal and smokeless cigarettes.
Previously, we have found that acetaldehyde, a volatile component of cigarette smoke, stimulates the protein kinase C (PKC) pathway and inhibits ciliary motility. A "smokeless" cigarette (Eclipse) now exists in which most of the tobacco is not burned, reducing the pyrolyzed components in the extract. We hypothesized that acetaldehyde is a component of cigarette smoke that activates PKC in the airway epithelial cell, and therefore the Eclipse cigarette would not activate epithelial cell PKC. In this study, bovine bronchial epithelial cells (BBEC) were incubated with cigarette smoke extract (CSE) or Eclipse smoke extract (ESE). We found that PKC activity was significantly higher in cells exposed to 5% CSE than cells exposed to 5% ESE or media. When acetaldehyde levels of both extracts were measured by gas chromatography, CSE was found to have 15-20 times greater concentration (microM) of acetaldehyde than ESE. When BBEC were treated with 5% CSE, ciliary beating was further decreased from baseline levels. This decrease in ciliary beating was not observed in cells treated with ESE, suggesting that acetaldehyde contained in CSE slows cilia. These results suggest that volatile components such as acetaldehyde in cigarette smoke may inhibit ciliary motility via a PKC-dependent mechanism.