在细胞和分子水平上调节人体肌肉收缩。

L Larsson, P Höök, P Pircher
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引用次数: 19

摘要

在肌肉收缩和肌原纤维蛋白异构体表达的调节方面,大鼠是最广泛表征的物种,但有理由质疑小型哺乳动物(如大鼠)的结果是否可以直接外推到大型哺乳动物(如人类)。人体肌肉收缩的研究主要使用不同的体内肌肉功能测量,即在电刺激或自主激活期间测量不同运动速度下的力。这些测量提供了整体肌肉功能的重要信息,但它们对我们理解肌肉收缩调节的价值有限。在基础科学中,细胞和分子生理学方法已经使用了很多年,但是这些技术到目前为止很少用于人体肌肉收缩的研究。人体肌肉收缩的详细研究可以在细胞和分子水平上通过经皮肌肉活检技术获得的短肌纤维段进行。皮纤维制剂与一种新型的体外运动测定相结合,提供了一种独特的可能性,可以在健康和疾病的同一肌肉细胞段中,即在根据表达的肌纤维蛋白同种异构体的类型和数量表征的肌肉细胞中,在细胞和分子水平上研究人体肌肉收缩的调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regulation of human muscle contraction at the cellular and molecular levels.

The rat is the most extensively characterized species with regard to regulation of muscle contraction and myofibrillar protein isoform expression, but there is reason to question whether results from small mammals, such as the rat, can be extrapolated directly to larger mammals, such as man. Studies of human muscle contraction have primarily used different in vivo muscle function measurements, i.e. measurements of force at different speeds of movement during electrical stimulation or voluntary activation. These measurements give important information on overall muscle function, but they are of limited value for our understanding of regulation of muscle contraction. In basic science, cellular- and molecular-physiological methods have been used for many years, but these techniques have so far only rarely been used in studies of human muscle contraction. Detailed studies of human muscle contraction can be performed in the short muscle fibre segments obtained by the percutaneous muscle biopsy technique both at the cellular and molecular level. The skinned fibre preparation in combination with a novel in vitro motility assay offers a unique possibility to investigate regulation of human muscle contraction at the cellular and molecular levels in the same muscle cell segment in both health and disease, i.e. in muscle cells characterized according to the type and amount of expressed myofibrillar protein isoforms.

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