P M Zeis, M Tzaki, L Nakopoulou, P Nicolaidou, E Kavazarakis, A Messaritaki, M Moustaki, M P Zeis, D Gourgiotis
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引用次数: 0
摘要
先前的研究人员一致认为，静脉注射叶酸(FA)会导致DNA合成增加和组织破坏。这项研究的目的是澄清由于细胞和细胞核的破坏导致的DNA降解是否先于FA处理后的DNA合成。40只豚鼠分为4组:1组，对照组10只;2组，10只动物腹腔注射300 mg/kg体wt FA;3组，10只动物注射FA, 12 h后肌注氟塞胺，剂量为7 mg/kg body wt;最后，在第4组，10只动物与第3组一样接受了氟塞胺治疗。DNA和胸腺嘧啶分解代谢的β -氨基异丁酸终产物检测表明，FA可引起曲小管上皮细胞坏死。3组给予氟脲胺对FA所致急性肾功能衰竭有良好的治疗效果。
DNA degradation in the kidney of folic acid-treated guinea pigs.
Previous investigators agree on the increased DNA synthesis and destruction of tissues caused by folic acid (FA) administered parenterally. This study aims to clarify whether DNA degradation due to the destruction of cells and nuclei precedes DNA synthesis following FA administration. Forty guinea pigs were divided into four groups: group 1, contained 10 controls; in group 2, ten animals received intraperitoneally 300 mg/kg of body wt FA; in group 3, ten animals received FA and 12 h later frusemide intramuscularly in a dose of 7 mg/kg body wt; and finally in group 4, ten animals received frusemide as in group 3. FA produced necrosis of the epithelial cells of the convoluted tubules as the detection of the beta-aminoisobutyric acid end product of DNA and thymine catabolism indicated. Frusemide administered in group 3 had a favourable effect on the acute renal failure induced by FA.
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