接触射频辉光放电聚合物诱导血小板粘附和促凝活性:吸附纤维蛋白原和vWF的作用。

J M Grunkemeier, W B Tsai, M R Alexander, D G Castner, T A Horbett
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引用次数: 49

摘要

采用体外血小板粘附和血小板催化凝血酶生成实验研究了六氟丙烯和乙烯(C(3)F(6)/C(2)H(4))或丙烯酸和1,7-辛二烯共聚制备的射频辉光放电(RFGD)聚合物的潜在血液相容性。凝血酶生成率归一化血小板数作为血小板活化(促凝活性)的测量。由丙烯酸和1,7 -辛二烯共聚制备的RFGD聚合物含有不同数量的羧酸,这是通过电子能谱(ESCA)进行化学分析的结果。这些聚合物在血小板粘附、凝血酶生成或血小板活化方面几乎没有引起变化。经ESCA测定,C(3)F(6)和C(2)H(4)的RFGD聚合产生的聚合物含不同比例的氟化物质。在饲料中添加25% C(3)F(6) (75% C(2)H(4))的聚合物上,血浆中纤维蛋白原的吸附量最大。然而,饲料中增加%C(3)F(6)的聚合物对冯氏血友病因子(vWF)的吸附更大。血小板粘附随着饲料中%C(3)F(6)的增加而降低。血小板粘附在由C(3)F(6)和C(2)H(4)制成的聚合物上凝血酶生成最低。因此,饲料中增加%C(3)F(6)的聚合物提高了血小板的促凝活性,与vWF吸附的趋势相似。这些发现表明,氟化物质在RFGD聚合物中的掺入增加导致血小板粘附减少和血小板活化增加(这可能是由于vWF吸附增加)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Platelet adhesion and procoagulant activity induced by contact with radiofrequency glow discharge polymers: roles of adsorbed fibrinogen and vWF.

The potential hemocompatibility of radiofrequency glow discharge (RFGD) polymers made by copolymerization of mixtures of hexafluoropropene and ethylene (C(3)F(6)/C(2)H(4)) or acrylic acid and 1,7-octadiene was investigated using in vitro assays for platelet adhesion and platelet catalyzed thrombin generation. Thrombin generation rate normalized to platelet number was used as a measurement of platelet activation (procoagulant activity). RFGD polymers produced by copolymerization of acrylic acid and 1, 7-octadiene contained varying amounts of carboxylic acid species as determined by electron spectroscopy for chemical analysis (ESCA). These polymers induced little variation in platelet adhesion, thrombin generation, or platelet activation. RFGD polymerization of C(3)F(6) and C(2)H(4) resulted in polymers with varying proportions of fluorinated species, as determined by ESCA. Fibrinogen adsorption from plasma was maximal on a polymer made with 25% C(3)F(6) (75% C(2)H(4)) in the feed. However von Willebrand factor (vWF) adsorption was greater on polymers made with increased %C(3)F(6) in the feed. Platelet adhesion decreased with increasing %C(3)F(6) in the feed. Thrombin generation was lowest for platelets adherent to polymers made from both C(3)F(6) and C(2)H(4). Therefore, procoagulant activity of platelets increased for polymers made with increased %C(3)F(6) in the feed, similar to the trend in vWF adsorption. These findings suggest that increased incorporation of fluorinated species into RFGD polymers leads to decreased platelet adhesion and increased platelet activation (which is possibly due to increased vWF adsorption).

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