土霉素对大鼠外分泌胰腺的影响。

C Lorenzo, M L del Olmo Martinez, L Pastor, A Almaraz, A Belmonte, A Caro-Patón
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引用次数: 5

摘要

目的:鉴于文献中描述的四环素引起的胰腺炎,我们研究了它对胰腺和十二指肠液酶含量以及胰腺超微结构的影响。在腺泡假说的背景下,我们评估了两性之间可能的差异,以及我们的发现与急性胰腺炎初始阶段所描述的结果之间的关系。方法:取Wistar大鼠128只(公63只,母65只),分为3组:对照组(I组),分别肌肉注射15、30 mg/kg/d土霉素(II、III组)。献祭前,每组各有一半大鼠接受胆囊收缩素刺激。麻醉后抽取血液、胰腺组织(用于酶剂量和形态学研究)和十二指肠液。结果:刺激III组雄鼠胰腺组织和十二指肠液淀粉酶水平明显降低(P < 0.003)。雌性老鼠的情况正好相反。其他酶(脂肪酶和胰蛋白酶)也有类似的趋势。在受刺激的动物中,酶原颗粒计数、未成熟颗粒的出现和胃浆的扩张更为频繁。结论:土霉素似乎能引起大鼠胰腺形态功能的改变,这种改变因性别而异。在女性中,酶积累可能会导致细胞内激活,并且在急性实验性胰腺炎的初始阶段也有超微结构的发现。这与文献中描述的接受四环素治疗的女性发生胰腺炎的更高频率一致。相比之下,对于雄性来说,这一发现与蛋白质合成的减少更为一致。这将使它们不容易受到吞噬现象的影响,因此,在腺泡或溶酶体假说的背景下,急性胰腺炎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of oxytetracycline on the rat exocrine pancreas.

Objectives: Given the appearance of pancreatitis attributed to tetracycline, as described in the literature, we have investigated its effect on the enzymatic content of pancreas and duodenal fluid and on pancreatic ultrastructure. We have evaluated possible differences between sexes and the relation of our findings with those described in the initial phases of acute pancreatitis, in the context of the acinar hypothesis.

Methods: With 128 Wistar rats (63 male and 65 female), 3 groups were established: control (group I) experimental animals treated with oxytetracycline intramuscularly, 15 and 30 mg/kg/d (groups II and III, respectively). Before sacrifice, half of the rats in each group were stimulated with cholecystokinin. Blood, pancreatic tissue (for enzyme dosage and morphological study), and duodenal fluid were extracted following anesthesia.

Results: The stimulated males of group III presented lower amylase levels in pancreatic tissue and duodenal fluid (P < 0.003). Just the opposite occurred in female rats. A similar tendency was observed with other enzymes (lipase and trypsin). Zymogen granule counts, appearance of immature granules, and dilation of ergastoplasm were more frequent in the stimulated animals.

Conclusions: Oxytetracycline seems to induce morphofunctional changes in rat pancreas, which differ according to sex. In the female, enzyme accumulation that could predispose intracellular activation seems to exist, as well as the ultrastructural findings described in initial phases of acute experimental pancreatitis. This agrees with the greater frequency of pancreatitis in women undergoing tetracycline treatment described in the literature. In contrast, for males the findings were more compatible with decrease of protein synthesis. This would make them less susceptible to crinophagy phenomena and, thus, to acute pancreatitis in the context of the acinar or lysosome hypothesis.

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