SSAO在人和牛脑膜和微血管中的存在。

Neurobiology (Budapest, Hungary) Pub Date : 1999-01-01

V Castillo, J M Lizcano, M Unzeta

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引用次数: 0

摘要

尽管SSAO酶广泛分布于几乎所有组织，特别是血管化组织，但其在脑微血管中的存在仍存在争议。我们的结果解决了这个问题，表明人和牛的脑血管组织都含有SSAO酶。这是通过生化方法实现的，使用苄胺和甲胺作为底物，并通过免疫印迹分析，使用抗ssao多克隆抗体，在组织匀浆中识别100 kDa的单带。

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Presence of SSAO in human and bovine meninges and microvessels.

In spite that SSAO enzyme is widely distributed in almost all tissues, specially in vascularized ones, its presence in brain microvessels is still controversy. Our results resolve this question showing that both human and bovine cerebrovascular tissues do contain the SSAO enzyme. This was achieved biochemically, using benzylamine and methylamine as substrates, and by immunoblot analysis, using polyclonal antibodies anti-SSAO that recognized a 100 kDa single band in tissue homogenates.

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来源期刊

Neurobiology (Budapest, Hungary)

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