拟南芥中的“双组分”乙烯信号传导。

C Chang, K Clark, X Wang, R Stewart
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引用次数: 0

摘要

拟南芥ETR1基因编码乙烯激素受体,该受体与细菌双组分调节因子具有惊人的序列相似性。这一发现预示着ETR1受体通过为许多细菌调节剂建立的磷传递机制来转导乙烯信号。为了验证这一假设,我们进行了ETR1自激酶活性以及向ETR1受体转移磷酸盐的体外实验。到目前为止,我们还没有检测到这两种活动。我们关注的另一个问题是ETR1受体的蛋白质底物的身份。利用酵母双杂交系统,我们获得了几个克隆,这些克隆随后被定性为ETR1的潜在相互作用体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
'Two-component' ethylene signaling in Arabidopsis.

The Arabidopsis ETR1 gene codes for an ethylene hormone receptor that has striking sequence similarity with bacterial two-component regulators. This finding predicts that the ETR1 receptor transduces the ethylene signal through the phosphotransfer mechanisms established for a number of the bacterial regulators. To test this hypothesis, we have performed in vitro assays for ETR1 autokinase activity as well as for transfer of phosphate to the ETR1 receiver. So far, we have not detected either of these activities. Another question we are focusing on is the identity of protein substrates of the ETR1 receptor. Using the yeast two-hybrid system, we have obtained several clones to be subsequently characterized as potential interactors with ETR1.

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