A Kittaka, T Kuze, M Amano, H Tanaka, T Miyasaka, K Hirose, T Yoshida, A Sarai, T Yasukawa, S Ishii
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Introduction of 6-formylcytidine into a Myb binding sequence.
Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 mers) as a substitute for thymidine in the Myb binding sequence of 3'-TTGAC-5'. The modified duplex showed Tm of 67 degrees C, which was six degrees lower than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and the binding was almost completely abolished.
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