[用去铁胺敏感性试验和海藻糖发酵试验鉴定表皮葡萄球菌]。

J Y Chiou, T M Wang, C H Chang, J J Wu, W C Tsai
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引用次数: 0

摘要

近年来,凝固酶阴性葡萄球菌,特别是表皮葡萄球菌在临床和医院感染中的重要性日益被认识到。因此,快速准确地鉴定表皮葡萄球菌是非常重要和必要的。一种新的测试,对去铁胺的敏感性,加上海藻糖发酵已被推荐用于鉴定这种生物。然而,使用的媒介和方法与NCCLS推荐的不同。为了探讨将去铁胺敏感性试验与常规的盘状agar扩散试验结合使用的可行性,我们采用111株葡萄球菌(包括51株表皮葡萄球菌,15株人型葡萄球菌和45株其他凝固酶阴性葡萄球菌)作为试验生物,并按照NCCLS推荐的程序使用muller - hinton agar和标准接种物。结果表明,1 mg去铁胺对表皮葡萄球菌和人型葡萄球菌均有抑制作用(抑制区直径为28 ~ 37 mm)。去铁胺对表皮葡萄球菌和人源葡萄球菌的最低抑菌浓度为125微克/毫升。人芽孢杆菌和表皮芽孢杆菌能够发酵海藻糖,使其进一步分化,前者能够发酵海藻糖,后者不能。结果表明,去铁胺对凝固酶阴性葡萄球菌的药敏试验可与常规的圆盘琼脂扩散法联合使用。表皮葡萄球菌对1 mg去铁胺敏感,不能发酵海藻糖,能快速准确地鉴定出表皮葡萄球菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Identification of Staphylococcus epidermidis by desferrioxamine susceptibility and trehalose fermentation tests].

The importance of coagulase-negative staphylococci, especially Staphylococcus epidermidis in clinical and nosocomial infection are recognized increasingly in recent years. A rapid and accurate identification of S. epidermidis is therefore important and necessary. A new test, susceptibility to desferrioxamine, coupled with trehalose fermentation has been recommended for the identification of this organism. However, the medium and method used are different from what has been recommended by the NCCLS. To investigate the feasibility of using the desferrioxamine susceptibility test in conjunction with the routinely used disc agar diffusion test, we employed 111 staphylococcal strains (including 51 S. epidermidis isolates, 15 S. hominis and 45 other coagulase-negative staphylococci) as test organisms, and followed the procedures recommended by the NCCLS in which Mueller-Hinton agar and standard inoculum were used. Results indicated that all strains of S. epidermidis and S. hominis were susceptible to 1 mg desferrioxamine (the diameter of the inhibition zone were 28-37 mm). The minimum inhibitory concentrations of desferrioxamine to S. epidermidis and S. hominis isolates were determined to be 125 micrograms/ml. Further differentiation of S. hominis and S. epidermidis can be made by their ability to ferment trehalose, the former could while the latter could not. We conclude that the desferrioxamine susceptibility test of coagulase-negative staphylococci can be used in conjunction with the routine disc agar diffusion method. S. epidermidis can be identified rapidly and accurately by its susceptibility to 1 mg desferrioxamine and inability to ferment trehalose.

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