{"title":"氮杂丁-2-羧酸对单次或分次x射线治疗肝癌细胞的影响以及对正常和耐热细胞的热致敏作用。","authors":"J van Rijn, J van den Berg, C A van der Mast","doi":"10.1002/(SICI)1520-6823(1999)7:5<270::AID-ROI2>3.0.CO;2-U","DOIUrl":null,"url":null,"abstract":"<p><p>The amino acid analog azetidine-2-carboxylic acid (azetidine) is a potent sensitizer to both hyperthermia and ionizing radiation. Incubation of H35 hepatoma cells with 2.5 mM azetidine before or after treatments with X-rays causes a time- and sequence-dependent enhancement of cell killing. Exposure of cells to 1-1.5 mM azetidine for 96 h in combination with repeated doses of 3 Gy X-rays at 24 h intervals causes an enhanced reduction of the surviving cell population due to both radiosensitization and an additional growth inhibition. Azetidine does not prevent the induction of thermotolerance after a heat shock. This thermotolerance proportionally reduces thermal radiosensitization but does not seem to affect azetidine radiosensitization. It is suggested that thermal radiosensitization and azetidine radiosensitization operate by different mechanisms.</p>","PeriodicalId":20894,"journal":{"name":"Radiation oncology investigations","volume":"7 5","pages":"270-7"},"PeriodicalIF":0.0000,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1520-6823(1999)7:5<270::AID-ROI2>3.0.CO;2-U","citationCount":"2","resultStr":"{\"title\":\"Effects of azetidine-2-carboxylic acid on treatments of hepatoma cells with single or fractionated X-ray irradiations and on thermal radiosensitization in normal and thermotolerant cells.\",\"authors\":\"J van Rijn, J van den Berg, C A van der Mast\",\"doi\":\"10.1002/(SICI)1520-6823(1999)7:5<270::AID-ROI2>3.0.CO;2-U\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The amino acid analog azetidine-2-carboxylic acid (azetidine) is a potent sensitizer to both hyperthermia and ionizing radiation. Incubation of H35 hepatoma cells with 2.5 mM azetidine before or after treatments with X-rays causes a time- and sequence-dependent enhancement of cell killing. Exposure of cells to 1-1.5 mM azetidine for 96 h in combination with repeated doses of 3 Gy X-rays at 24 h intervals causes an enhanced reduction of the surviving cell population due to both radiosensitization and an additional growth inhibition. Azetidine does not prevent the induction of thermotolerance after a heat shock. This thermotolerance proportionally reduces thermal radiosensitization but does not seem to affect azetidine radiosensitization. It is suggested that thermal radiosensitization and azetidine radiosensitization operate by different mechanisms.</p>\",\"PeriodicalId\":20894,\"journal\":{\"name\":\"Radiation oncology investigations\",\"volume\":\"7 5\",\"pages\":\"270-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/(SICI)1520-6823(1999)7:5<270::AID-ROI2>3.0.CO;2-U\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Radiation oncology investigations\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/(SICI)1520-6823(1999)7:5<270::AID-ROI2>3.0.CO;2-U\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiation oncology investigations","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/(SICI)1520-6823(1999)7:5<270::AID-ROI2>3.0.CO;2-U","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
氨基酸类似物氮杂啶-2-羧酸(氮杂啶)是对高温和电离辐射的有效敏化剂。在x射线治疗前后用2.5 mM azetidine孵育H35肝癌细胞,可引起细胞杀伤的时间和序列依赖性增强。将细胞暴露于1-1.5 mM氮杂啶96小时,同时每隔24小时进行3 Gy x射线的重复剂量,由于放射致敏和额外的生长抑制,导致存活细胞数量的增加。Azetidine不能阻止热休克后耐热性的诱导。这种耐热性按比例降低热放射致敏性,但似乎不影响氮杂啶的放射致敏性。热辐射敏化和氮杂啶辐射敏化的作用机制不同。
Effects of azetidine-2-carboxylic acid on treatments of hepatoma cells with single or fractionated X-ray irradiations and on thermal radiosensitization in normal and thermotolerant cells.
The amino acid analog azetidine-2-carboxylic acid (azetidine) is a potent sensitizer to both hyperthermia and ionizing radiation. Incubation of H35 hepatoma cells with 2.5 mM azetidine before or after treatments with X-rays causes a time- and sequence-dependent enhancement of cell killing. Exposure of cells to 1-1.5 mM azetidine for 96 h in combination with repeated doses of 3 Gy X-rays at 24 h intervals causes an enhanced reduction of the surviving cell population due to both radiosensitization and an additional growth inhibition. Azetidine does not prevent the induction of thermotolerance after a heat shock. This thermotolerance proportionally reduces thermal radiosensitization but does not seem to affect azetidine radiosensitization. It is suggested that thermal radiosensitization and azetidine radiosensitization operate by different mechanisms.