替拉帕嗪、碳与烟酰胺增强人类肿瘤移植对分级辐照反应的效果比较。

A el-Said, D Menke, M J Dorie, J M Brown
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引用次数: 7

摘要

本研究的目的是比较人类肿瘤异种移植,两种不同的策略来增加肿瘤对分次照射的反应,即用碳和烟酰胺氧合缺氧肿瘤细胞,或用缺氧细胞毒素替拉帕胺(TPZ)杀死这些细胞。我们将人下咽鳞状细胞癌FaDu细胞株植入免疫缺陷SCID小鼠体内,通过细胞存活和生长延迟来评估其对辐射的反应。用TPZ (0.08 mmol/kg)或烟酰胺(1,000 mg/kg)进行碳呼吸照射,每日1次或2次,剂量分别为2或2.5 Gy/次。我们还测试了隔天给TPZ的效果,或者在疗程的前半段每天给TPZ,后半段每天给TPZ,或者在整个放疗过程中给TPZ。我们发现,在分级放疗方案中加入TPZ或烟酰胺与碳可以增强人类异种移植物的反应。TPZ的增强程度更大(尽管不是很明显),特别是每次放射剂量时。综上所述,在分次辐照中加入TPZ或烟酰胺加碳,增强了该人类肿瘤异种移植物对分次辐照的反应。与理论模型一致的是,每次放射剂量给予TPZ时,肿瘤的放射反应比只给予一半放射剂量时增强得更大。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of the effectiveness of tirapazamine and carbogen with nicotinamide in enhancing the response of a human tumor xenograft to fractionated irradiation.

The goal of this study was to compare, with a human tumor xenograft, two different strategies for increasing tumor response to fractionated irradiation, namely, oxygenating the hypoxic tumor cells with carbogen and nicotinamide, or killing these cells with the hypoxic cytotoxin, tirapazamine (TPZ). We used the human hypopharyngeal squamous cell carcinoma cell line FaDu implanted in immune-deficient SCID mice and assessed its response to radiation by cell survival and by growth delay. The tumors were irradiated either once or twice daily with 2 or 2.5 Gy/fraction with either TPZ (0.08 mmol/kg) or nicotinamide (1,000 mg/kg) with carbogen breathing. We also tested the effect of giving TPZ on alternate days, or daily during the first half of the course, the second half, or for the whole course of radiation. We found that adding TPZ or nicotinamide with carbogen to the fractionated radiation regimen enhanced the response of the human xenograft. The enhancement was somewhat greater (though not significantly so) for TPZ, especially when given with each radiation dose. In conclusion, adding TPZ, or nicotinamide plus carbogen, to fractionated irradiation enhanced the response of this human tumor xenograft to fractionated irradiation. Consistent with theoretical modeling, there was a greater enhancement of the radiation response of the tumor when TPZ was given with each radiation dose than when given with only half of the radiation doses.

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