评估人类胶质瘤细胞对顺铂、热疗和放疗反应的交叉耐药,并选择8个顺铂耐药克隆。

G P Raaphorst, D E Wilkins, J P Mao, J C Miao, C E Ng
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引用次数: 3

摘要

人类胶质瘤细胞暴露于逐步增加浓度的顺铂,并给予最终,急性,高浓度的顺铂治疗。从存活细胞中选择8个顺铂耐药克隆。这些克隆显示出一系列顺铂敏感性,当细胞连续传代时,这些敏感性在没有顺铂的情况下保持不变。这些细胞被测试对辐射和42度和45度高温的交叉抗性。数据显示,8个克隆中有7个比亲本系更耐辐射,而一个对辐射更敏感。顺铂耐药程度与放射线耐药程度无关。对于42摄氏度和45摄氏度的高温,一些克隆比亲本系的抵抗力略强,而一个克隆则敏感得多。这不是对辐射敏感的克隆体。总之,顺铂耐药、放疗耐药和热疗反应之间没有直接的相关性,尽管一些克隆对所有三种治疗都耐药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of cross-resistance between responses to cisplatin, hyperthermia, and radiation in human glioma cells and eight clones selected for cisplatin resistance.

Human glioma cells were exposed to stepwise increasing concentrations of cisplatin and given a final, acute, high concentration treatment of cisplatin. From the surviving cells, eight cisplatin resistant clones were selected. These clones demonstrated a range of cisplatin sensitivities that were retained in the absence of cisplatin when cells were continually passaged. These cells were tested for cross-resistance to radiation and hyperthermia at 42 and 45 degrees C. The data showed that seven of the eight clones were also more radioresistant than the parental line, while one was more radiosensitive. The degree of cisplatin resistance was not related to the degree of radiation resistance. For hyperthermia at 42 and 45 degrees C, some of the clones were slightly more resistant than the parental line, while one clone was much more sensitive. This was not the same clone that was radiosensitive. In conclusion, there was no direct correlation between cisplatin resistance, radiation resistance, and hyperthermia response, although some of the clones were resistant to all three treatments.

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