极低频电容耦合弱电场对6B1细胞代谢的影响

Bioelectrochemistry and bioenergetics (Lausanne, Switzerland) Pub Date : 1999-05-01 DOI:10.1016/S0302-4598(99)00024-0

Xing Wang , Aihua Zhou , Man Liu , Haiying Yu , Lijun Pang , Mingyao Zhu , Leiguang Wang , Hermann Berg

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引用次数: 4

摘要

本研究采用MTT比色法、DAPI荧光法、ELISA等多种方法，研究了极低频(ELF)电容耦合电场(EFs)对6B1细胞代谢的影响。结果表明，50 mV cm−1 ELF EF (10-100 Hz)对6B1细胞的增殖、DNA合成和琥珀酸脱氢酶活性无显著影响，说明ELF (10-100 Hz) EF对6B1细胞代谢的影响不明显。而50 mV cm−1,50 Hz EF显著促进6B1细胞的HBs-Ab(乙型肝炎表面抗体)分泌，说明在这种情况下，EF对6B1细胞膜外表面有一定的特殊作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Effects of ELF capacitively coupled weak electric fields on metabolism of 6B1 cells

In this study, we adopted several methods of MTT colorimetry, DAPI fluorimetry and ELISA to study the effects of extremely low frequency (ELF) capacitively coupled electric fields (EFs) on the metabolism of 6B1 cells. The result shows that 50 mV cm⁻¹ ELF EF (10–100 Hz) has no significant effect on proliferation, DNA synthesis and activity of succinate dehydrogenase of 6B1 cells, indicating that the effect of ELF (10–100 Hz) EF on the metabolism of 6B1 cells is not obvious. However, 50 mV cm⁻¹, 50 Hz EF significantly promotes the HBs-Ab (Hepatitis B surface antibody) secretion of 6B1 cells, implying that under this situation, EF has some distinctive effect on the outerface of 6B1 cell membrane.

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Bioelectrochemistry and bioenergetics (Lausanne, Switzerland)

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