{"title":"完整红细胞在人足月胎盘羊膜上的多脉冲介导电融合","authors":"Subrata Biswas, Sujoy K Guha","doi":"10.1016/S0302-4598(99)00042-2","DOIUrl":null,"url":null,"abstract":"<div><p>The creation of surface modified human term placental amnion by electrofusing human cells onto its surface has been thought of. A multiple-pulse electrofusion protocol with 10 square pulses of 10-μs pulse length, and electric field of 0.2 kV cm<sup>−1</sup>, can make erythrocyte–amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion onto human tissue with minimal procedural complexities.</p></div>","PeriodicalId":79804,"journal":{"name":"Bioelectrochemistry and bioenergetics (Lausanne, Switzerland)","volume":"48 2","pages":"Pages 431-434"},"PeriodicalIF":0.0000,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0302-4598(99)00042-2","citationCount":"0","resultStr":"{\"title\":\"Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion\",\"authors\":\"Subrata Biswas, Sujoy K Guha\",\"doi\":\"10.1016/S0302-4598(99)00042-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The creation of surface modified human term placental amnion by electrofusing human cells onto its surface has been thought of. A multiple-pulse electrofusion protocol with 10 square pulses of 10-μs pulse length, and electric field of 0.2 kV cm<sup>−1</sup>, can make erythrocyte–amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion onto human tissue with minimal procedural complexities.</p></div>\",\"PeriodicalId\":79804,\"journal\":{\"name\":\"Bioelectrochemistry and bioenergetics (Lausanne, Switzerland)\",\"volume\":\"48 2\",\"pages\":\"Pages 431-434\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0302-4598(99)00042-2\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioelectrochemistry and bioenergetics (Lausanne, Switzerland)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0302459899000422\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectrochemistry and bioenergetics (Lausanne, Switzerland)","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0302459899000422","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Multiple-pulse-mediated electrofusion of intact erythrocyte onto human term placental amnion
The creation of surface modified human term placental amnion by electrofusing human cells onto its surface has been thought of. A multiple-pulse electrofusion protocol with 10 square pulses of 10-μs pulse length, and electric field of 0.2 kV cm−1, can make erythrocyte–amnion tissue electrofusion possible. The protocol devised merge the cell-tissue-adherence steps with fusogenic pulse. The finding opens up a new avenue of cell electrofusion onto human tissue with minimal procedural complexities.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
