人胰腺导管腺癌12q22-q24.1胸腺嘧啶- dna糖基酶基因的基因组分析。

T Yatsuoka, T Furukawa, T Abe, T Yokoyama, M Sunamura, M Kobari, S Matsuno, A Horii
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引用次数: 17

摘要

结论:胸腺嘧啶- dna糖基酶(TDG)基因12q22-q24.1的异常在胰腺导管癌的发生中可能起有限的作用。背景:最近发现了一种人类G/ t特异性TDG基因。这种蛋白质在一个系统中起着纠正G/T错配到G/C对的作用。TDG被定位到染色体带12q22-q24.1,这是胰腺癌中经常丢失的区域之一。因此,12q上的TDG基因有可能是导致胰腺导管癌发生的基因之一。方法:对21株人胰腺癌细胞系TDG基因全编码区核苷酸序列进行分析。通过Northern杂交分析了TDG基因在几种人体组织和21种人胰腺癌细胞系中的mRNA表达。结果:胰腺癌细胞系mRNA表达水平降低,但未见体细胞突变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genomic analysis of the thymine-DNA glycosylase (TDG) gene on 12q22-q24.1 in human pancreatic ductal adenocarcinoma.

Conclusion: Abnormality of the thymine-DNA glycosylase (TDG) gene on 12q22-q24.1 appears to play a limited role in pancreatic ductal carcinogenesis.

Background: Recently, a human G/T-specific TDG gene was identified. This protein acts in a system correcting G/T mispairs to G/C pairs. TDG was mapped to chromosome bands 12q22-q24.1, one of the regions frequently lost in pancreatic cancer. Therefore, there is the possibility that the TDG gene on 12q is one of the genes responsible for pancreatic ductal carcinogenesis.

Methods: Nucleotide sequences of the entire coding region of the TDG gene were analyzed in 21 human pancreatic cancer cell lines. mRNA expression of the TDG gene was also analyzed by Northern hybridization in several human tissues and 21 human pancreatic cancer cell lines.

Results: Decreased levels of mRNA expression were detected in the pancreatic cancer cell lines, but no somatic mutations were observed.

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