l -去戊烯醇诱导大鼠黑质和腹侧被盖芳香族l -氨基酸脱羧酶mRNA表达。原位杂交研究。

X M Li, A V Juorio, J Qi, A A Boulton
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引用次数: 9

摘要

l -去戊烯醇是一种复杂的药物,人们提出了许多机制来解释其作用。这些包括阻断多巴胺代谢,放大多巴胺反应,诱导超氧化物歧化酶或延迟细胞凋亡。利用原位杂交技术,我们发现l -去戊烯醇(5-10 mg/kg腹腔注射,24 h后杀死)增加了大鼠下丘脑外盖区芳香烃l -氨基酸脱羧酶(AADC) mRNA水平。在人脑组织中,AADC存在于低水平,提示可能在单胺合成中起限速作用。这在帕金森患者中尤为重要,因为左旋多巴的治疗效果归因于其酶促多巴胺脱羧作用。目前的研究结果支持左旋去戊烯醇的作用之一可能是通过增加AADC的可用性来促进左旋多巴的脱羧。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
L-deprenyl induces aromatic L-amino acid decarboxylase (AADC) mRNA in the rat substantia nigra and ventral tegmentum. An in situ hybridization study.

L-Deprenyl is a complex drug, and number of mechanisms have been proposed to explain its effects. These include blockade of dopamine metabolism, amplification of dopamine responses, induction of superoxide dismutase or delaying apoptosis. Using in situ hybridization techniques, we have shown that L-deprenyl (5-10 mg/kg intraperitoneally, killed after 24 h) increases aromatic L-amino acid decarboxylase (AADC) mRNA levels in rat substantia nigraventral tegmental area. In human brain tissue, AADC is present at low levels, suggesting a possible rate-limiting role in monoamine synthesis. This is particularly important in parkinsonian patients, since the therapeutic efficacy of L-DOPA is attributed to its enzymatic decarboxylation to dopamine. The present findings support that one of the effects of L-deprenyl may be to facilitate the decarboxylation of L-DOPA by increasing the availability of AADC.

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