IM小鼠脑内瘙痒剂87V对儿茶酚胺能神经元的广泛变性。

S W Yun, E K Choi, W K Ju, M S Ahn, R I Carp, H M Wisniewski, Y S Kim
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引用次数: 13

摘要

痒病是绵羊和山羊中枢神经系统的一种退行性疾病。这种病原体已经传播到许多实验室物种,包括老鼠和仓鼠。淀粉样斑块的形成和空泡化,老年性痴呆的迹象,被发现在小鼠大脑感染87V痒病剂。采用高效液相色谱-电化学检测器(HPLC-ECD)测定瘙痒病感染小鼠脑内多巴胺(DA)和去甲肾上腺素(NE)的浓度。NE水平在所有测试区域均显著下降,而DA水平仅在大脑皮层显著下降。应用酪氨酸羟化酶(TH)抗血清免疫组化检测黑质和蓝斑的儿茶酚胺神经元的免疫反应。与对照组相比,瘙痒病感染小鼠黑质和蓝斑中th免疫反应神经元的数量显著减少。这些数据表明,去甲肾上腺素能和多巴胺能系统对痒病剂87V的作用都很敏感,而瘙痒病感染小鼠大脑中儿茶酚胺水平的变化可能导致了一些临床症状,如共济失调和失用症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extensive degeneration of catecholaminergic neurons to scrapie agent 87V in the brains of IM mice.

Scrapie is a degenerative disease of the central nervous system of sheep and goats. The causative agent has been passaged to a number of laboratory species, including mice and hamster. Amyloid plaque formation and vacuolation, the signs of senile dementia, are found in the brains of mice infected with 87V scrapie agent. Dopamine (DA) and norepinephrine (NE) concentrations in the brains of scrapie-infected mice were measured with high-performance liquid chromatography-electrochemical detector (HPLC-ECD). A significant decrease in NE level was exhibited in all regions tested, whereas the level of DA decreased significantly only in cerebral cortex. Immunohistochemistry was used to examine immunoreactive catecholamine neurons in substantia nigra and locus ceruleus using antisera against tyrosine hydroxylase (TH). The population of TH-immunoreactive neurons in the substantia nigra and locus ceruleus were significantly decreased in scrapie-infected mice compared to controls. These data suggest that both the noradrenergic and dopaminergic system are sensitive to the action of scrapie agent 87V and that changes in the catecholamine levels in the brains of scrapie-infected mice may contribute to some of the clinical symptoms of the diseases, such as ataxia and apraxia.

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