{"title":"eb - barr病毒携带细胞中EBER-1和EBER-2的差异表达和定位。","authors":"N Teramoto, L Szekely, G Klein","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The functions of the Epstein-Barr virus (EBV)-encoded small RNAs (EBER-1 and EBER-2) are unknown. We examined their fine intranuclear localization as the first step to investigate their function.</p><p><strong>Methods: </strong>We analyzed EBER-1 and EBER-2 by in situ hybridization combined with two-color immunofluorescence tagging.</p><p><strong>Results: </strong>EBER-1 was visualized as fine dots, mainly in the euchromatin. Ribosomal protein L-22 also formed fine dots, similar to those formed by EBER-1, in the nuclei of EBV-carrying cells and colocalized with the latter by double staining. EBER-2 was predominantly found in the nucleoli and was also present in the euchromatin. The two EBERs were similarly expressed in B-cell lines of the different phenotypes examined. The EBERs showed no colocalization by double staining. Image analysis indicated that the level of their expression was not correlated.</p><p><strong>Conclusion: </strong>The differential localization and expression of the EBER-1 and EBER-2 suggests that they may play different functional roles.</p>","PeriodicalId":80032,"journal":{"name":"Journal of human virology","volume":"1 5","pages":"307-13"},"PeriodicalIF":0.0000,"publicationDate":"1998-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differential expression and localization of EBER-1 and EBER-2 in Epstein-Barr virus-carrying cells.\",\"authors\":\"N Teramoto, L Szekely, G Klein\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The functions of the Epstein-Barr virus (EBV)-encoded small RNAs (EBER-1 and EBER-2) are unknown. We examined their fine intranuclear localization as the first step to investigate their function.</p><p><strong>Methods: </strong>We analyzed EBER-1 and EBER-2 by in situ hybridization combined with two-color immunofluorescence tagging.</p><p><strong>Results: </strong>EBER-1 was visualized as fine dots, mainly in the euchromatin. Ribosomal protein L-22 also formed fine dots, similar to those formed by EBER-1, in the nuclei of EBV-carrying cells and colocalized with the latter by double staining. EBER-2 was predominantly found in the nucleoli and was also present in the euchromatin. The two EBERs were similarly expressed in B-cell lines of the different phenotypes examined. The EBERs showed no colocalization by double staining. Image analysis indicated that the level of their expression was not correlated.</p><p><strong>Conclusion: </strong>The differential localization and expression of the EBER-1 and EBER-2 suggests that they may play different functional roles.</p>\",\"PeriodicalId\":80032,\"journal\":{\"name\":\"Journal of human virology\",\"volume\":\"1 5\",\"pages\":\"307-13\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of human virology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of human virology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Differential expression and localization of EBER-1 and EBER-2 in Epstein-Barr virus-carrying cells.
Objective: The functions of the Epstein-Barr virus (EBV)-encoded small RNAs (EBER-1 and EBER-2) are unknown. We examined their fine intranuclear localization as the first step to investigate their function.
Methods: We analyzed EBER-1 and EBER-2 by in situ hybridization combined with two-color immunofluorescence tagging.
Results: EBER-1 was visualized as fine dots, mainly in the euchromatin. Ribosomal protein L-22 also formed fine dots, similar to those formed by EBER-1, in the nuclei of EBV-carrying cells and colocalized with the latter by double staining. EBER-2 was predominantly found in the nucleoli and was also present in the euchromatin. The two EBERs were similarly expressed in B-cell lines of the different phenotypes examined. The EBERs showed no colocalization by double staining. Image analysis indicated that the level of their expression was not correlated.
Conclusion: The differential localization and expression of the EBER-1 and EBER-2 suggests that they may play different functional roles.
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