上皮细胞与成骨水泥细胞共定位形成非细胞骨质,但与成骨水泥细胞不共定位形成细胞骨质。

Acta anatomica Pub Date : 1998-01-01 DOI:10.1159/000046440
M Kagayama, Y Sasano, J Zhu, M Hirata, I Mizoguchi, S Kamakura
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引用次数: 20

摘要

使用抗层粘连蛋白抗体和/或花生凝集素(PNA)和抗骨钙素(OC)抗体分别检测了大鼠磨牙根形成早期阶段马拉塞氏上皮细胞和成水泥细胞。第一磨牙的牙根是用来研究的。抗层粘连蛋白抗体染色上皮根鞘和上皮细胞周围的基底膜。上皮根鞘的基膜是连续的,而上皮基膜是不连续的。上皮细胞和上皮根鞘细胞PNA阳性。在PNA和抗层粘连蛋白抗体双重染色的切片中可以看到上皮细胞的结构特征。2周龄大鼠上皮细胞呈成纤维细胞样,呈细网状结构。3周龄大鼠根表面冠状半面也有上皮组织,呈现典型的细胞束,而根表面根尖部分细胞牙骨质覆盖着牙本质,未见上皮组织。在3周龄大鼠的根尖处,上皮细胞在上皮根鞘附近形成细网。抗oc抗体染色骨水泥母细胞内衬脱细胞和细胞骨水泥。用抗oc和抗层粘连蛋白抗体或PNA双重染色的切片进一步揭示了上皮细胞与成水泥细胞之间的密切关系。在脱细胞牙骨质或牙本质内的oc阳性细胞定位在离上皮细胞非常近的地方。相比之下,细胞骨质衬层的oc阳性细胞与上皮细胞没有密切的联系,除了位于最顶端的细胞,在那里上皮根鞘的基膜被破坏,开始形成最初的细胞骨质。目前的研究结果表明,上皮细胞和/或它们的不连续基底膜可能至少在根形成的早期阶段对脱细胞牙骨质的形成起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epithelial rests colocalize with cementoblasts forming acellular cementum but not with cementoblasts forming cellular cementum.

Epithelial rests of Malassez and cementoblasts were examined in the rat molars during the early stages of root formation using an antilaminin antibody and/or peanut agglutinin (PNA), and an antiosteocalcin (OC) antibody, respectively. The roots of the first molars were used for study. The antilaminin antibody stained the basement membrane surrounding the epithelial root sheath and epithelial rests. The basement membrane of the epithelial root sheath was continuous, but that of the epithelial rests was discontinuous. The cells of epithelial rests and epithelial root sheath were positive for PNA. The structural characteristics of the epithelial rests were seen in the sections stained doubly with PNA and the antilaminin antibody. The cells of epithelial rests were fibroblast-like and formed a fine mesh in 2-week-old rats. In 3-week-old rats, the epithelial rests were also present at the coronal half of root surface, showing typical cell cords, but were not present at the apical part of the root surface where the cellular cementum covered the root dentin. At the root apex of 3-week-old rats, the cells of epithelial rests forming fine meshes were seen near the epithelial root sheath. The anti-OC antibody stained cementoblasts lining acellular and cellular cementum. The sections doubly stained with the anti-OC and the antilaminin antibodies or PNA further revealed the close relation between epithelial rests and cementoblasts. The OC-positive cells lining acellular cementum or dentin were localized very close to the epithelial rests. In contrast, the OC-positive cells lining cellular cementum did not show close association with the epithelial cells, except the cells located most apically where the basement membrane of the epithelial root sheath is disrupted and the initial cellular cementum begins to be formed. The present results suggest that the epithelial rests and/or the discontinuous basement membrane of them may have a role for the acellular cementum formation at least in the early stage of root formation.

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