高剂量率、低剂量率和高剂量率分割辐射的比较,以优化体外放射敏感性差异。

R C Wilkins, C E Ng, G P Raaphorst
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引用次数: 15

摘要

放射治疗的实施假设所有患者对放射的反应相似,尽管每个患者的放射敏感性确实不同,导致不同程度的早期和晚期效应。由于给病人的剂量受到治疗现场正常组织反应的限制,因此在治疗前确定正常组织的敏感性是有益的。先前预测放射敏感性的研究使用了单次体外剂量后的存活部分,然而,在这种低杀伤水平下细胞存活的差异并不容易解决。在这项研究中,我们开始评估替代剂量方案的使用,这可能更好地解决放射敏感性的差异。我们研究了几种具有预测价值的辐射方案,包括高剂量(6 Gy)、高剂量(112 cGy/min)和低剂量(112 cGy/min)后的生存。882 cGy/min)剂量率和2 Gy(6次)分次剂量后。从治疗后表现出严重影响的患者身上培养的敏感人成纤维细胞系(S11358)与从明显正常受试者身上培养的细胞系(OMB1)进行了比较。将这些细胞系与两种分别对体外辐射表现出耐药和敏感反应的人类黑色素瘤细胞系(SKMEL3和HT144)之间的差异进行了比较。在成纤维细胞和黑色素瘤细胞系中,无论是低剂量率、高剂量率还是分次剂量照射,正常细胞和敏感细胞的存活率差异都随着剂量的增加而增加。我们建议更接近模拟临床治疗的辐射剂量比2 Gy (SF2)后的存活分数更适合于体外评估细胞群的相对放射敏感性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of high dose rate, low dose rate, and high dose rate fractionated radiation for optimizing differences in radiosensitivities in vitro.

Radiotherapy is administered with the assumption that all patients respond similarly to radiation although radiosensitivity does vary from patient to patient, resulting in different degrees of early and late effects. Because the dose given to a patient is limited by the response of normal tissue in the treatment field, it would be beneficial to determine the sensitivity of this normal tissue prior to therapy. Previous studies to predict radiosensitivity have used surviving fractions after a single dose given in vitro, however, differences in cell survival at this low level of kill are not easy to resolve. In this study, we set out to evaluate the use of alternative dose regimens which may better resolve differences in radiosensitivity. We have examined several radiation protocols for predictive value, including survival after high doses (6 Gy) at both high (112 cGy/min) and low (.882 cGy/min) dose rates and after fractionated doses of 2 Gy (6 fractions). A sensitive human fibroblast line (S11358) cultured from a patient showing severe effects after therapy is compared with a cell line (OMB1) cultured from an apparently normal subject. Differences between these cell lines have been compared with those between two human melanoma cell lines (SKMEL3 and HT144) which have shown resistant and sensitive response to radiation in vitro respectively. In both fibroblast and melanoma cell lines, the difference in the survival of normal and sensitive cells increased with increasing dose regardless of whether irradiation was delivered as low dose rate, high dose rate, or as fractionated doses. We propose that radiation doses which more closely mimic clinical treatment are more suitable than surviving fraction after 2 Gy (SF2) for in vitro evaluation of relative radiosensitivities of cell populations.

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