抗氧化基因NKEF在中枢神经系统中的表达。

T A Sarafian, C Huang, A Kim, J de Vellis, H Shau
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引用次数: 11

摘要

自由基及其造成的氧化应激会对神经系统造成严重伤害,并导致与各种退行性和创伤性疾病相关的病理。在这项研究中,我们使用Western和Northern blot分析检测了抗氧化防御基因nkef在人组织和分离的大鼠脑细胞中的表达。NKEF蛋白在人脑、肝脏、肾脏、肌肉和肺中均有表达。人内皮细胞系ECV在正常观察到的22 kda条带之外表达了25 kda条带。在中枢神经系统中,22 kda的NKEF条带存在于皮层灰质和白质、海马、小脑和脊髓中,其数量大致相似。NKEF-A和NKEF-B亚型的表达通过Northern分析的胚胎大鼠脑培养细胞类型来评估。星形胶质细胞和小胶质细胞同时表达22和25 kda蛋白带,而皮质神经元和少突胶质细胞仅含有22 kda蛋白带。这些细胞类型的Northern blot分析显示,NKEF-A在神经元和少突胶质细胞中的表达水平较低,而NKEF-B在小胶质细胞中的表达水平相对较低。这些抗氧化防御基因的差异表达可能有助于脑细胞类型对特定种类氧化应激的选择性脆弱性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of the antioxidant gene NKEF in the central nervous system.

Free radicals and the oxidative stress they impose can cause serious injury in the nervous system and contribute to pathology associated with a wide variety of degenerative and traumatic disorders. In this study, we examined the expression of an antioxidant defense gene, nkef, in human tissue and isolated populations of rat brain cells using Western and Northern blot analysis. NKEF protein was expressed in human brain, liver, kidney, muscle, and lung. The human endothelial cell line ECV expressed a 25-kDa band in addition to the 22-kDa band normally observed. In the central nervous system, a 22-kDa NKEF band was present in cortical gray and white matter, hippocampus, cerebellum, and spinal cord in roughly similar amounts. Expression of NKEF-A and NKEF-B subtypes was evaluated by Northern analysis of cultured cell types from embryonic rat brain. Astrocyte and microglia expressed both 22- and 25-kDa bands, whereas cortical neurons and oligodendrocytes contained only the 22-kDa protein band. Northern blot analysis of these cell types revealed low levels of NKEF-A message in neurons and oligodendrocytes, and relatively low levels of NKEF-B in microglia. Differential expression of these antioxidant defense genes may contribute to the selective vulnerability of brain cell types to specific kinds of oxidative stress.

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