腹膜B-1细胞中存在种系和全长IgA RNA转录本。

R de Waard, P M Dammers, J W Tung, A B Kantor, J A Wilshire, N A Bos, L A Herzenberg, F G Kroese
{"title":"腹膜B-1细胞中存在种系和全长IgA RNA转录本。","authors":"R de Waard,&nbsp;P M Dammers,&nbsp;J W Tung,&nbsp;A B Kantor,&nbsp;J A Wilshire,&nbsp;N A Bos,&nbsp;L A Herzenberg,&nbsp;F G Kroese","doi":"10.1155/1998/37576","DOIUrl":null,"url":null,"abstract":"<p><p>Next to conventional B cells (or B-2 cells), peritoneal B-1 cells have been shown to contribute significantly to the production of IgA-secreting plasma cells in the gut. Evidence for this was mainly based on studies comprising manipulated animals, including lethally X-irradiated and transgenic mice. To examine the ability of peritoneal B-1 cells from untreated mice to switch actively to IgA in vivo, we performed RT-PCR analysis on FACS-sorted peritoneal B-cell subsets from untreated BALB/c mice in order to examine the presence of germline C alpha mRNA and mature C alpha mRNA transcripts. Germline C alpha and mature C alpha transcripts were readily detectable in peritoneal B-1 cells (defined as IgMbright/IgDdull), but not, or very little, in peritoneal B-2 cells (defined as IgMdull/IgDbright). Moreover, by subdividing the B-1-cell population in CD5+ B-1a cells and CD5- B-1b cells, it was shown that in vivo expression of germline C alpha and mature C alpha transcripts was largely restricted to the B-1b-cell lineage. These results indicate that peritoneal B-1 cells indeed are capable to switch to IgA under normal physiological conditions and hereby further support the view that B-1 cells contribute significantly to the mucosal IgA response, albeit this function appears to be restricted to the B-1b-cell subset.</p>","PeriodicalId":77106,"journal":{"name":"Developmental immunology","volume":"6 1-2","pages":"81-7"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/1998/37576","citationCount":"32","resultStr":"{\"title\":\"Presence of germline and full-length IgA RNA transcripts among peritoneal B-1 cells.\",\"authors\":\"R de Waard,&nbsp;P M Dammers,&nbsp;J W Tung,&nbsp;A B Kantor,&nbsp;J A Wilshire,&nbsp;N A Bos,&nbsp;L A Herzenberg,&nbsp;F G Kroese\",\"doi\":\"10.1155/1998/37576\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Next to conventional B cells (or B-2 cells), peritoneal B-1 cells have been shown to contribute significantly to the production of IgA-secreting plasma cells in the gut. Evidence for this was mainly based on studies comprising manipulated animals, including lethally X-irradiated and transgenic mice. To examine the ability of peritoneal B-1 cells from untreated mice to switch actively to IgA in vivo, we performed RT-PCR analysis on FACS-sorted peritoneal B-cell subsets from untreated BALB/c mice in order to examine the presence of germline C alpha mRNA and mature C alpha mRNA transcripts. Germline C alpha and mature C alpha transcripts were readily detectable in peritoneal B-1 cells (defined as IgMbright/IgDdull), but not, or very little, in peritoneal B-2 cells (defined as IgMdull/IgDbright). Moreover, by subdividing the B-1-cell population in CD5+ B-1a cells and CD5- B-1b cells, it was shown that in vivo expression of germline C alpha and mature C alpha transcripts was largely restricted to the B-1b-cell lineage. These results indicate that peritoneal B-1 cells indeed are capable to switch to IgA under normal physiological conditions and hereby further support the view that B-1 cells contribute significantly to the mucosal IgA response, albeit this function appears to be restricted to the B-1b-cell subset.</p>\",\"PeriodicalId\":77106,\"journal\":{\"name\":\"Developmental immunology\",\"volume\":\"6 1-2\",\"pages\":\"81-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/1998/37576\",\"citationCount\":\"32\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/1998/37576\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental immunology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/1998/37576","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 32

摘要

与常规B细胞(或B-2细胞)相比,腹膜B-1细胞已被证明对肠道中分泌iga的浆细胞的产生有重要贡献。这方面的证据主要是基于对操纵动物的研究,包括致命的x射线照射和转基因小鼠。为了检测未经处理的小鼠腹膜B-1细胞在体内主动转换为IgA的能力,我们对未经处理的BALB/c小鼠的facs分类腹膜b细胞亚群进行了RT-PCR分析,以检测种系c α mRNA和成熟c α mRNA转录物的存在。生殖系C α和成熟C α转录本在腹膜B-1细胞(定义为IgMbright/ igdull)中很容易检测到,但在腹膜B-2细胞(定义为igmull /IgDbright)中检测不到或很少。此外,通过细分CD5+ B-1a细胞和CD5- B-1b细胞中的b- 1细胞群,我们发现种系C - α和成熟C - α转录本在体内的表达主要局限于B-1b细胞系。这些结果表明,腹膜B-1细胞确实能够在正常生理条件下转换为IgA,从而进一步支持B-1细胞对粘膜IgA反应有重要贡献的观点,尽管这种功能似乎仅限于b- 1b细胞亚群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Presence of germline and full-length IgA RNA transcripts among peritoneal B-1 cells.

Next to conventional B cells (or B-2 cells), peritoneal B-1 cells have been shown to contribute significantly to the production of IgA-secreting plasma cells in the gut. Evidence for this was mainly based on studies comprising manipulated animals, including lethally X-irradiated and transgenic mice. To examine the ability of peritoneal B-1 cells from untreated mice to switch actively to IgA in vivo, we performed RT-PCR analysis on FACS-sorted peritoneal B-cell subsets from untreated BALB/c mice in order to examine the presence of germline C alpha mRNA and mature C alpha mRNA transcripts. Germline C alpha and mature C alpha transcripts were readily detectable in peritoneal B-1 cells (defined as IgMbright/IgDdull), but not, or very little, in peritoneal B-2 cells (defined as IgMdull/IgDbright). Moreover, by subdividing the B-1-cell population in CD5+ B-1a cells and CD5- B-1b cells, it was shown that in vivo expression of germline C alpha and mature C alpha transcripts was largely restricted to the B-1b-cell lineage. These results indicate that peritoneal B-1 cells indeed are capable to switch to IgA under normal physiological conditions and hereby further support the view that B-1 cells contribute significantly to the mucosal IgA response, albeit this function appears to be restricted to the B-1b-cell subset.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信