{"title":"利用GFAP-lacZ转基因小鼠测定胚胎腹侧中脑移植物中星形胶质细胞的命运","authors":"Quintana, Lopez-Colberg, Cunningham","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Embryonic ventral midbrains from GFAP-lacZ transgenic mice were xenografted into the dopamine-depleted striata of adult rats. This transgenic line harbors a nuclear-targeted bacterial beta-galactosidase (beta-gal) reporter gene under transcriptional control of the human glial fibrillary acidic protein (GFAP) promoter sequence. Five weeks post-transplantation, graft-derived astrocytes and dopaminergic neurons were visualized by dual immunocytochemistry for beta-gal and tyrosine hydroxylase (TH), respectively. This report describes the advantages associated with the use of GFAP-lacZ transgenic mice to study astrocyte fate in embryonic neural grafts.</p>","PeriodicalId":9057,"journal":{"name":"Brain research. Developmental brain research","volume":"105 1","pages":"147-51"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Use of GFAP-lacZ transgenic mice to determine astrocyte fate in grafts of embryonic ventral midbrain\",\"authors\":\"Quintana, Lopez-Colberg, Cunningham\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Embryonic ventral midbrains from GFAP-lacZ transgenic mice were xenografted into the dopamine-depleted striata of adult rats. This transgenic line harbors a nuclear-targeted bacterial beta-galactosidase (beta-gal) reporter gene under transcriptional control of the human glial fibrillary acidic protein (GFAP) promoter sequence. Five weeks post-transplantation, graft-derived astrocytes and dopaminergic neurons were visualized by dual immunocytochemistry for beta-gal and tyrosine hydroxylase (TH), respectively. This report describes the advantages associated with the use of GFAP-lacZ transgenic mice to study astrocyte fate in embryonic neural grafts.</p>\",\"PeriodicalId\":9057,\"journal\":{\"name\":\"Brain research. Developmental brain research\",\"volume\":\"105 1\",\"pages\":\"147-51\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brain research. Developmental brain research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brain research. Developmental brain research","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Use of GFAP-lacZ transgenic mice to determine astrocyte fate in grafts of embryonic ventral midbrain
Embryonic ventral midbrains from GFAP-lacZ transgenic mice were xenografted into the dopamine-depleted striata of adult rats. This transgenic line harbors a nuclear-targeted bacterial beta-galactosidase (beta-gal) reporter gene under transcriptional control of the human glial fibrillary acidic protein (GFAP) promoter sequence. Five weeks post-transplantation, graft-derived astrocytes and dopaminergic neurons were visualized by dual immunocytochemistry for beta-gal and tyrosine hydroxylase (TH), respectively. This report describes the advantages associated with the use of GFAP-lacZ transgenic mice to study astrocyte fate in embryonic neural grafts.
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