C Helige, G Zellnig, R Hofmann-Wellenhof, R Fink-Puches, J Smolle, H A Tritthart
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引用次数: 0
摘要
在肿瘤侵袭的复杂过程中,细胞间通讯和恶性细胞进入和通过宿主组织屏障的积极运动起着至关重要的作用。比较了体内良性和恶性黑色素细胞的运动活性、细胞骨架肌动蛋白和血管蛋白组织以及间隙连接通讯,并将其与体外侵袭性进行了比较。与高度转移的黑色素瘤细胞K1735-M2相比,正常黑色素细胞Melan-a表现出明显更低的运动活性、更高的肌动蛋白细胞骨架组织和更多含血管素的细胞基质粘附斑块。在相同的培养条件下,间隙连接交际无显著差异。然而,在含有肿瘤启动子12- o - tetradecanoylphorol -13-acetate (TPA)的传统黑素细胞生长培养基中培养melana细胞可增强细胞间通讯。在鸡胚心脏模型和Matrigel侵袭实验中,黑素细胞的侵袭性都小于黑素细胞。在缺乏TPA的培养基中培养的黑素细胞具有最小的侵袭活性,表明补充TPA的培养基刺激了侵袭。两种黑素细胞系的某些体外特性的比较表明,运动性与体外侵袭呈正相关,而肌动蛋白丝组织程度和血管蛋白斑块数量呈负相关。间隙连接通讯与体外侵袭性无直接关系。
Interrelation of motility, cytoskeletal organization and gap junctional communication with invasiveness of melanocytic cells in vitro.
Intercellular communication and the active movement of malignant cells into and through host tissue barriers play a critical role during the complex process of tumor invasion. Motile activity, cytoskeletal actin and vinculin organization as well as gap junctional communication of in vivo benign and malignant melanocytes were compared and related to in vitro invasiveness. Normal melanocytes, Melan-a, showed significantly less motile activity, a higher organization of the actin cytoskeleton and more vinculin-containing cell-substratum adhesion plaques than highly metastatic melanoma cells, K1735-M2. There was no pronounced difference in gap junctional communication under comparable culture conditions. However, cultivation of Melan-a cells in a conventional melanocyte growth medium containing the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) enhanced intercellular communication. Melanocytes were less invasive than melanoma cells both in the embryonic chick heart model and in the Matrigel invasion assay. The least invasive activity was determined for melanocytes cultivated in TPA-deficient medium indicating that the medium supplement TPA stimulates invasion. The comparison of certain in vitro properties of both melanocytic cell lines revealed a positive correlation of motility with in vitro invasion, whereas an inverse correlation was found for the degree of actin filament organization as well as for the number of vinculin plaques. Gap junctional communication was not directly related to in vitro invasiveness.