大鼠肝移植腺癌的间质性激光热疗。

P H Möller, K Ivarsson, U Stenram, M Radnell, K G Tranberg
{"title":"大鼠肝移植腺癌的间质性激光热疗。","authors":"P H Möller,&nbsp;K Ivarsson,&nbsp;U Stenram,&nbsp;M Radnell,&nbsp;K G Tranberg","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To examine the effect of different temperatures and exposure times in interstitial laser thermotherapy.</p><p><strong>Design: </strong>Controlled laboratory study.</p><p><strong>Setting: </strong>University hospital, Sweden.</p><p><strong>Material: </strong>48 male Wistar FU rats with dimethylhydrazine-induced adenocarcinoma transplanted into the liver.</p><p><strong>Intervention: </strong>Treatment was given with an Nd:YAG laser and a feedback system for temperature regulation. Light was delivered into the centre of the tumour and the feedback thermistor probe was placed 3 mm from the tumour margin. Rats were treated at steady-state temperatures at the feedback thermistor of 43, 46, or 50 degrees C for 30 minutes, and at a steady-state temperature of 46 degrees C at the feedback thermistor also for 10 and 20 minutes.</p><p><strong>Main outcome measurement: </strong>Tumour control as assessed 6 days after treatment using light microscopical examination including immunohistochemical determination of bromodeoxyuridine (BrdU) incorporation into DNA as a measure of cell viability.</p><p><strong>Results: </strong>Complete tumour necrosis was achieved in all rats treated for 30 minutes, in 6/8 rats treated for 10 minutes and in 6/8 rats treated for 20 minutes at 46 degrees C. During steady-state thermotherapy, temperatures at the tumour margin were about 11 degrees higher than at the feedback thermistor (range 54-61 degrees C). The surrounding liver tissue also became necrotic so that the total necrosis volume exceeded the pretreatment tumour volume.</p><p><strong>Conclusion: </strong>Interstitial laser thermotherapy at temperatures ranging from 54-61 degrees C at the tumour margin ensures total necrosis of a transplanted rat liver carcinoma provided that treatment is given for 30 minutes.</p>","PeriodicalId":22411,"journal":{"name":"The European journal of surgery = Acta chirurgica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Interstitial laser thermotherapy of adenocarcinoma transplanted into rat liver.\",\"authors\":\"P H Möller,&nbsp;K Ivarsson,&nbsp;U Stenram,&nbsp;M Radnell,&nbsp;K G Tranberg\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To examine the effect of different temperatures and exposure times in interstitial laser thermotherapy.</p><p><strong>Design: </strong>Controlled laboratory study.</p><p><strong>Setting: </strong>University hospital, Sweden.</p><p><strong>Material: </strong>48 male Wistar FU rats with dimethylhydrazine-induced adenocarcinoma transplanted into the liver.</p><p><strong>Intervention: </strong>Treatment was given with an Nd:YAG laser and a feedback system for temperature regulation. Light was delivered into the centre of the tumour and the feedback thermistor probe was placed 3 mm from the tumour margin. Rats were treated at steady-state temperatures at the feedback thermistor of 43, 46, or 50 degrees C for 30 minutes, and at a steady-state temperature of 46 degrees C at the feedback thermistor also for 10 and 20 minutes.</p><p><strong>Main outcome measurement: </strong>Tumour control as assessed 6 days after treatment using light microscopical examination including immunohistochemical determination of bromodeoxyuridine (BrdU) incorporation into DNA as a measure of cell viability.</p><p><strong>Results: </strong>Complete tumour necrosis was achieved in all rats treated for 30 minutes, in 6/8 rats treated for 10 minutes and in 6/8 rats treated for 20 minutes at 46 degrees C. During steady-state thermotherapy, temperatures at the tumour margin were about 11 degrees higher than at the feedback thermistor (range 54-61 degrees C). The surrounding liver tissue also became necrotic so that the total necrosis volume exceeded the pretreatment tumour volume.</p><p><strong>Conclusion: </strong>Interstitial laser thermotherapy at temperatures ranging from 54-61 degrees C at the tumour margin ensures total necrosis of a transplanted rat liver carcinoma provided that treatment is given for 30 minutes.</p>\",\"PeriodicalId\":22411,\"journal\":{\"name\":\"The European journal of surgery = Acta chirurgica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The European journal of surgery = Acta chirurgica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The European journal of surgery = Acta chirurgica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的:探讨不同温度和照射时间对间质性激光热疗的影响。设计:实验室对照研究。地点:瑞典大学医院。材料:48只雄性Wistar FU大鼠二甲肼诱导腺癌肝移植。干预:给予Nd:YAG激光和温度调节反馈系统治疗。将光送入肿瘤中心,反馈热敏电阻探头放置在距肿瘤边缘3mm处。大鼠在反馈热敏电阻43、46或50℃的稳态温度下处理30分钟,在反馈热敏电阻46℃的稳态温度下处理10和20分钟。主要结果测量:治疗后6天使用光镜检查评估肿瘤控制,包括免疫组织化学测定溴脱氧尿苷(BrdU)并入DNA作为细胞活力的测量。结果:所有大鼠在46℃下治疗30分钟,6/8大鼠治疗10分钟,6/8大鼠在46℃下治疗20分钟,肿瘤完全坏死。稳态热疗时,肿瘤边缘温度比反馈热敏电阻温度(54 ~ 61℃)高约11℃,周围肝组织也开始坏死,坏死总量超过预处理肿瘤体积。结论:肿瘤边缘54-61℃的间隙性激光热疗法,只要治疗30分钟,就能保证移植的大鼠肝癌完全坏死。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interstitial laser thermotherapy of adenocarcinoma transplanted into rat liver.

Objective: To examine the effect of different temperatures and exposure times in interstitial laser thermotherapy.

Design: Controlled laboratory study.

Setting: University hospital, Sweden.

Material: 48 male Wistar FU rats with dimethylhydrazine-induced adenocarcinoma transplanted into the liver.

Intervention: Treatment was given with an Nd:YAG laser and a feedback system for temperature regulation. Light was delivered into the centre of the tumour and the feedback thermistor probe was placed 3 mm from the tumour margin. Rats were treated at steady-state temperatures at the feedback thermistor of 43, 46, or 50 degrees C for 30 minutes, and at a steady-state temperature of 46 degrees C at the feedback thermistor also for 10 and 20 minutes.

Main outcome measurement: Tumour control as assessed 6 days after treatment using light microscopical examination including immunohistochemical determination of bromodeoxyuridine (BrdU) incorporation into DNA as a measure of cell viability.

Results: Complete tumour necrosis was achieved in all rats treated for 30 minutes, in 6/8 rats treated for 10 minutes and in 6/8 rats treated for 20 minutes at 46 degrees C. During steady-state thermotherapy, temperatures at the tumour margin were about 11 degrees higher than at the feedback thermistor (range 54-61 degrees C). The surrounding liver tissue also became necrotic so that the total necrosis volume exceeded the pretreatment tumour volume.

Conclusion: Interstitial laser thermotherapy at temperatures ranging from 54-61 degrees C at the tumour margin ensures total necrosis of a transplanted rat liver carcinoma provided that treatment is given for 30 minutes.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信