溶组织内阿米巴的基因扩增。

Invasion & metastasis Pub Date : 1996-01-01
M Báez-Camargo, R Gharaibeh, A M Riverón, F de la Cruz Hernández, J P Luna, P Gariglio, P Chávez, E Orozco
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引用次数: 0

摘要

我们在这里展示的数据表明,导致人类阿米巴病的原生动物溶组织内阿米巴,以一种类似于转化的哺乳动物细胞的方式呈现DNA扩增。通过透射电子显微镜(TEM),我们发现线性、圆形和同心圆形DNA分子表现出非预定DNA扩增过程的主要事件。在两个不相邻的DNA区域重组后形成环,并且在不涉及环出序列的情况下从重组链中出现气泡。气泡长大了,经过进一步的复制,形成了一套部分复制的圆圈。多环复合体也从假定的复制起源形成,没有重组远端DNA区域。从菌株HM1:IMSS衍生的克隆显示出不同的DNA含量,表明DNA扩增。亲本克隆A和子代克隆C2在rDNA基因拷贝数上存在差异,但这种差异仅在脉冲场凝胶电泳分离总DNA时出现,而在核DNA上没有发现显著差异。对TEM观察到的事件进行解剖,使我们提出了一种洋葱皮模型,用于溶组织芽胞杆菌的基因扩增。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gene amplification in Entamoeba histolytica.

We show here data suggesting that Entamoeba histolytica, the protozoan responsible for human amebiasis, presents DNA amplification in a fashion similar to that described for transformed mammalian cells. By transmission electron microscopy (TEM), we found linear, circular and concentric circular DNA molecules exhibiting the main events of the unscheduled DNA amplification process. Loops were formed after the recombination of two nonadjacent DNA regions, and bubbles appeared from the recombinant strands without involving the looped-out sequences. Bubbles grew up and underwent further replication rounds to produce a nested set of partially replicated circles. Multicircle complexes were also formed from putative replication origin without recombination of distant DNA regions. Clones derived from the strain HM1:IMSS exhibited different DNA contents, suggesting DNA amplification. The parental clone A and its daughter clone C2 differed in rDNA gene copy numbers, but this was observed only when total DNA was separated by pulse field gel electrophoresis, and no significant differences were detected in nuclear DNA. The dissection of the events observed by TEM led us to propose an onion skin model for gene amplification in E. histolytica.

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