槟榔碱对口腔黏膜成纤维细胞的细胞毒和细胞抑制作用。

C L Tsai, M Y Kuo, L J Hahn, Y S Kuo, P J Yang, J H Jeng
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引用次数: 0

摘要

在台湾,嚼槟榔液与口腔黏膜下纤维化及口腔癌的发生有密切关系。槟榔碱是槟榔的主要生物碱,被认为是BQ的病因之一。为了阐明槟榔碱在BQ咀嚼相关口腔黏膜病变发病机制中的作用,我们利用口腔黏膜成纤维细胞研究血清浓度、细胞密度和孵育时间对槟榔碱细胞毒性反应的影响。当浓度小于0.2 mM时,槟榔碱在1、3和6天后对口腔粘膜细胞没有细胞毒性。孵育3天后,当细胞暴露于高浓度槟榔碱(0.2 mM)和血清(10% FCS)时,槟榔碱的细胞毒和细胞抑制作用明显。细胞(1 × 10(4)个细胞/孔)在0.5% FCS中暴露于0.2 mM槟榔碱3天和6天,细胞数量分别减少20%和23%,而细胞(1 × 10(4)个细胞/孔)在10% FCS中暴露于0.2 mM槟榔碱,细胞数量分别减少38%和53%。在较高的细胞密度(5 × 10(4)和1 × 10(5)个细胞/孔)下,0.2 mM槟油碱在孵育6天后导致细胞毒性降低(细胞数量分别减少38%和21%)。结果表明槟榔碱对口腔黏膜成纤维细胞无丝分裂作用,槟榔碱对口腔黏膜成纤维细胞的细胞毒性和细胞抑制作用可能受细胞密度、血清浓度和培养时间的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxic and cytostatic effects of arecoline on oral mucosal fibroblasts.

Betel quid (BQ) chewing shows strong correlation to the incidence of oral submucous fibrosis and oral cancer in Taiwan. Arecoline, the main areca alkaloid, is considered to be one of the etiologic factors in BQ. To elucidate the role(s) of arecoline in the pathogenesis of BQ chewing related oral mucosal lesions, we used oral mucosal fibroblasts to study the effects of serum concentration, cell density, and incubation time on the cytotoxic response to arecoline. At a concentration less than 0.2 mM, arecoline was not cytotoxic to oral mucosal cells after 1, 3, and 6 days of incubation. After 3 days of incubation, the cytotoxic and cytostatic effects of arecoline became evident when the cells were exposed to higher concentrations of arecoline (0.2 mM) and serum (10% FCS). Exposure of cells (1 x 10(4) cells/well) to 0.2 mM of arecoline in 0.5% FCS for 3 and 6 days led to a 20% and 23% decrease, respectively, in the cell number, whereas exposure of cells (1 x 10(4) cells/well) to 0.2 mM arecoline in 10% FCS led to a 38% and 53% decrease, respectively, in cell number. At a higher cell density (5 x 10(4) and 1 x 10(5) cells/well), 0.2 mM arecoline led to less cytotoxicity (38% and 21% of decreasing in cell number, respectively) after 6 days of incubation. Our results indicated that arecoline was not mitogenic to oral mucosal fibroblasts, and that the cytotoxic and cytostatic effects of arecoline on oral mucosal fibroblasts could be modulated by the changes in the cell density, serum concentrations, and incubation time.

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