{"title":"内皮素通过ETA受体在培养的人颗粒叶黄素细胞中抑制fsh介导的功能。","authors":"C Furger, J R Zorn, F Ferré","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Endothelins (ETs) are a family of vasoactive peptides involved in granulosa and luteal cell function in some animal species. However, the potential relevance of ETs in ovarian physiology remains unclear, and the direct action of the peptides in the human ovary has not been studied to date. Experiments were conducted to determine whether ET-1 and ET-3 could regulate the follicle stimulating hormone (FSH)-induced cell response in human granulosa-lutein cells in culture. The FSH-mediated cell rounding process was used as an indicator of cell response, as previously described (Lawrence et al., 1979). Forskolin, cholera toxin, 8-Br-cyclic AMP, isobutylmethylxanthine (IBMX), rolipram and FSH all stimulated similar cell morphological changes, indicating that the cell rounding process was mediated by cyclic AMP. Although ET-1 and ET-3 alone failed to alter cell shape, the FSH-induced cell response was totally inhibited by treatment with ET-1 (10(-10) mol/l) and ET-3 (10(-7) mol/l). In addition, treatment of the cells with BQ123, an antagonist of ET binding on ETA receptor subtype, totally prevented the inhibitory effects of ET-1 and ET-3 on the FSH-induced response. The data presented here show that human granulosalutein cells are a site of ET reception and action. Endothelins inhibit cyclic AMP-dependent FSH-mediated function and the ET(A) receptor participates in this effect.</p>","PeriodicalId":11444,"journal":{"name":"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy","volume":"1 3","pages":"188-95"},"PeriodicalIF":0.0000,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Endothelins inhibit FSH-mediated function via ETA receptors in cultured human granulosa-lutein cells.\",\"authors\":\"C Furger, J R Zorn, F Ferré\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Endothelins (ETs) are a family of vasoactive peptides involved in granulosa and luteal cell function in some animal species. However, the potential relevance of ETs in ovarian physiology remains unclear, and the direct action of the peptides in the human ovary has not been studied to date. Experiments were conducted to determine whether ET-1 and ET-3 could regulate the follicle stimulating hormone (FSH)-induced cell response in human granulosa-lutein cells in culture. The FSH-mediated cell rounding process was used as an indicator of cell response, as previously described (Lawrence et al., 1979). Forskolin, cholera toxin, 8-Br-cyclic AMP, isobutylmethylxanthine (IBMX), rolipram and FSH all stimulated similar cell morphological changes, indicating that the cell rounding process was mediated by cyclic AMP. Although ET-1 and ET-3 alone failed to alter cell shape, the FSH-induced cell response was totally inhibited by treatment with ET-1 (10(-10) mol/l) and ET-3 (10(-7) mol/l). In addition, treatment of the cells with BQ123, an antagonist of ET binding on ETA receptor subtype, totally prevented the inhibitory effects of ET-1 and ET-3 on the FSH-induced response. The data presented here show that human granulosalutein cells are a site of ET reception and action. Endothelins inhibit cyclic AMP-dependent FSH-mediated function and the ET(A) receptor participates in this effect.</p>\",\"PeriodicalId\":11444,\"journal\":{\"name\":\"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy\",\"volume\":\"1 3\",\"pages\":\"188-95\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
内皮素(ETs)是一个血管活性肽家族,在一些动物物种中参与颗粒和黄体细胞功能。然而,ETs在卵巢生理学中的潜在相关性尚不清楚,并且肽在人类卵巢中的直接作用至今尚未研究。通过实验研究ET-1和ET-3是否能调节促卵泡激素(FSH)诱导的人颗粒叶黄素细胞的细胞反应。如前所述,fsh介导的细胞舍入过程被用作细胞反应的指标(Lawrence et al., 1979)。弗斯可林、霍乱毒素、8- br -环AMP、异丁基甲基黄嘌呤(IBMX)、罗利普兰和FSH均刺激了类似的细胞形态变化,表明细胞圆切过程是由环AMP介导的。虽然ET-1和ET-3单独不能改变细胞形状,但ET-1 (10(-10) mol/l)和ET-3 (10(-7) mol/l)处理完全抑制了FSH诱导的细胞反应。此外,用ET结合ETA受体亚型的拮抗剂BQ123处理细胞,完全阻止ET-1和ET-3对fsh诱导反应的抑制作用。这里的数据显示,人颗粒蛋白细胞是ET接受和作用的一个部位。内皮素抑制环amp依赖性fsh介导的功能,ET(A)受体参与了这一作用。
Endothelins inhibit FSH-mediated function via ETA receptors in cultured human granulosa-lutein cells.
Endothelins (ETs) are a family of vasoactive peptides involved in granulosa and luteal cell function in some animal species. However, the potential relevance of ETs in ovarian physiology remains unclear, and the direct action of the peptides in the human ovary has not been studied to date. Experiments were conducted to determine whether ET-1 and ET-3 could regulate the follicle stimulating hormone (FSH)-induced cell response in human granulosa-lutein cells in culture. The FSH-mediated cell rounding process was used as an indicator of cell response, as previously described (Lawrence et al., 1979). Forskolin, cholera toxin, 8-Br-cyclic AMP, isobutylmethylxanthine (IBMX), rolipram and FSH all stimulated similar cell morphological changes, indicating that the cell rounding process was mediated by cyclic AMP. Although ET-1 and ET-3 alone failed to alter cell shape, the FSH-induced cell response was totally inhibited by treatment with ET-1 (10(-10) mol/l) and ET-3 (10(-7) mol/l). In addition, treatment of the cells with BQ123, an antagonist of ET binding on ETA receptor subtype, totally prevented the inhibitory effects of ET-1 and ET-3 on the FSH-induced response. The data presented here show that human granulosalutein cells are a site of ET reception and action. Endothelins inhibit cyclic AMP-dependent FSH-mediated function and the ET(A) receptor participates in this effect.